Immunometabolic features of the formation of post-vaccination immunity against porcine circovirus type 2 in sows

Relevance. Immunometabolic status plays an important role in the formation of post-vaccination immunity against porcine circovirus type 2 in sows.
Methods. The object of the study was sows that were vaccinated with the “Ingelvac CircoFLEX” vaccine (Germany) on the 21st day of lactation after weaning their piglets (control group). In the experimental group, vaccination was combined with the administration of “Transfer Factor” obtained from leukocytes of hyperimmunized animals. The effectiveness of vaccination was assessed by parameters of immunometabolic status and production indicators.
Results. The introduction of “Transfer Factor” into the vaccination scheme of sows against pig circovirus of the second type makes it possible to form an immunometabolism profile in the animals› body, promoting the production of virus-neutralizing antibodies in the required quantity, which is reflected in the value of production and economically important indicators as markers of the effectiveness of postvaccination immunity. This is achieved due to the fact that post-vaccination immunological reactions occur predominantly through the mechanism of a secondary immune response, as evidenced by an increase in the concentration of IgG by 1.46–1.55 times and a decrease in IgM by 1.63–2.11 times, compared with the control. The hepatoprotective properties of “Transfer Factor” modulate the functional ability of liver cells and stabilize the state of their membrane structures, which determines the orientation of protein and lipid metabolism in the body of sows in an anabolic direction, promoting the retention of protein nitrogen and the accumulation of reserve fats in the body of animals, the use of carbon residues of amino acids in the Krebs cycle through the regulation of the activity of transamination enzymes (AlAT, AST), control of the choleretic ability of hepatocytes, rational cholesterol metabolism. Correction of the immunometabolism status of sows in the post-vaccination period allows, in comparison with the control, to reduce the retirement of sows from the pig farm population by 21.05%, the stillbirth of piglets by 38.15%, increasing the number of adopted ones by 10.55%, and increasing the yield of piglets by 1 farrowing. 12.5 heads to 13 and their safety at farrowing is 0.80%.

1. Cheng C., Wei H., Yu H., Xu C., Jiang S., Peng J. Metabolic Syndrome During Perinatal Period in Sows and the Link With Gut Microbiota and Metabolites. Frontiers in Microbiology. 2018; 9: 1989. https://doi.org/10.3389/fmicb.2018.01989

2. Kramareva I.A., Kramarev I.V., Semenyutin V.V. Metabolic profile of blood of sows in different physiological states in case of implementation of various biologically active substances. Belgorod State University Scientific Bulletin. Natural Sciences. 2017; 25: 91–98 (in Russian). https://elibrary.ru/zxhhah

3. Nair R.R., Verma P., Singh K. Immuneendocrine crosstalk during pregnancy. General and Comparative Endocrinology. 2017; 242: 18–23. https://doi.org/10.1016/j.ygcen.2016.03.003

4. Liu B. et al. Consumption of Dietary Fiber from Different Sources during Pregnancy Alters Sow Gut Microbiota and Improves Performance and Reduces Inflammation in Sows and Piglets. mSystems. 2021; 6(1): e00591-20. https://doi.org/10.1128/mSystems.00591-20

5. Thum C. et al. Can Nutritional Modulation of Maternal Intestinal Microbiota Influence the Development of the Infant Gastrointestinal Tract?. The Journal of Nutrition. 2012; 142(11): 1921–1928. https://doi.org/10.3945/jn.112.166231

6. Song H. et al. Effects of Dietary Monoglyceride and Diglyceride Supplementation on the Performance, Milk Composition, and Immune Status of Sows During Late Gestation and Lactation. Frontiers in Veterinary Science. 2021; 8: 714068. https://doi.org/10.3389/fvets.2021.714068

7. Mor G., Aldo P., Alvero A.B. The unique immunological and microbial aspects of pregnancy. Nature Reviews Immunology. 2017; 17(8): 469–482. https://doi.org/10.1038/nri.2017.64

8. Cox S.L., O’Siorain J.R., Fagan L.E., Curtis A.M., Carroll R.G. Intertwining roles of circadian and metabolic regulation of the innate immune response. Seminars in Immunopathology. 2022; 44(2): 225–237. https://doi.org/10.1007/s00281-021-00905-5

9. Mai J. et al. High Co-infection Status of Novel Porcine Parvovirus 7 With Porcine Circovirus 3 in Sows That Experienced Reproductive Failure. Frontiers in Veterinary Science. 2021; 8: 695553. https://doi.org/10.3389/fvets.2021.695553

10. Burkov P.V., Scherbakov P.N., Derkho M.A., Rebezov M.B. Aspects of the formation of post-vaccination immunity against porcine circovirus infection and its correction. Agrarian science. 2022; 10: 32–37 (in Russian). https://doi.org/10.32634/0869-8155-2022-363-10-32-37

11. Burkov P.V. et al. Pathological features of the lungs and liver of piglets under conditions of constant vaccination of livestock against circovirus infection. Theory and practice of meat processing. 2023; 8(1): 4–11. https://doi.org/10.21323/2414-438X-2023-8-1-4-11

12. Brigadirov Yu.N., Kotsarev V.N., Shaposhnikov I.T., Lobanov A.E., Falkova Yu.O. Some Indicators of Immuno-Biochemical Status of Sows in Case of Inflammatory Processes in Reproductive Organs. Russian veterinary journal. 2018; 1: 9–11 (in Russian). https://elibrary.ru/yvwcbo

13. Burkov P.V., Derkho M.A., Rebezov M.B., Shcherbakov P.N., Derkho A.O., Stepanova K.V. Immunological status of sows during the reproductive cycle and correction of its condition with an antigen-directed biostimulator. Agrarian science. 2023; 12: 58–66 (in Russian). https://doi.org/10.32634/0869-8155-2023-377-12-58-66

14. Burkov P.V., Derkho M.A., Rebezov M.B., Scherbakov P.N. Circovirus as a factor controlling the effectiveness of pregnancy in sows. Agrarian science. 2023; 8: 27–35 (in Russian). https://doi.org/10.32634/0869-8155-2023-373-8-27-35

15. Stafford V.V. Second type of pigs’ circovirus infection. Russian Journal of Agricultural and Socio-Economic Sciences. 2017; 5: 306–309 (in Russian). https://doi.org/10.18551/rjoas.2017-05.39

16. Zhao H. et al. Damage of zona pellucida reduces the developmental potential and quality of porcine circovirus type 2-infected oocytes after parthenogenetic activation. Theriogenology. 2014; 82(6): 790–799. https://doi.org/10.1016/j.theriogenology.2014.06.003

17. Hansen S. et al. Detection of porcine cytomegalovirus, a roseolovirus, in pig ovaries and follicular fluid: implications for somatic cells nuclear transfer, cloning and xenotransplantation. Virology Journal. 2023; 20: 15. https://doi.org/10.1186/s12985-023-01975-7

18. Bielanski A., Algire J., Lalonde A., Garceac A., Pollard J.W., Plante C. Nontransmission of porcine circovirus 2 (PCV2) by embryo transfer. Theriogenology. 2013; 80(2): 77–83. https://doi.org/10.1016/j.theriogenology.2013.03.022

19. Tummaruk P., Pearodwong P. Expression of PCV2 antigen in the ovarian tissues of gilts. Journal of Veterinary Medical Science. 2016; 78(3): 457–461. https://doi.org/10.1292/jvms.15-0450

20. Uribe-García H.F., Suarez-Mesa R.A., Rondón-Barragán I.S. Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia. Veterinary Medicine and Science. 2022; 8(6): 2451–2459. https://doi.org/10.1002/vms3.949

21. Raev S.А. Vaccination against porcine circovirus diseases: the present state and future prospects. Russian veterinary journal. 2014; 1: 26–29 (in Russian). https://elibrary.ru/sahywn

22. Guo J. et al. Porcine Circovirus Type 2 Vaccines: Commercial Application and Research Advances. Viruses. 2022; 14(9): 2005. https://doi.org/10.3390/v14092005

23. Kim K., Choi K., Shin M., Hahn T.-W. A porcine circovirus type 2d-based virus-like particle vaccine induces humoral and cellular immune responses and effectively protects pigs against PCV2d challenge. Frontiers in Microbiology. 2024; 14: 1334968. https://doi.org/10.3389/fmicb.2023.1334968

24. Noh Y.-H. et al. Pathological Evaluation of Porcine Circovirus 2d (PCV2d) Strain and Comparative Evaluation of PCV2d and PCV2b Inactivated Vaccines against PCV2d Infection in a Specific Pathogen-Free (SPF) Yucatan Miniature Pig Model. Vaccines. 2022; 10(9): 1469. https://doi.org/10.3390/vaccines10091469

25. Deng Y. et al. A novel strategy for an anti-idiotype vaccine: nanobody mimicking neutralization epitope of porcine circovirus type 2. Journal of Virology. 2024; 98(2): e01650-23. https://doi.org/10.1128/jvi.01650-23

26. Li Q. et al. Maternal Nutrition During Late Gestation and Lactation: Association With Immunity and the Inflammatory Response in the Offspring. Frontiers in Immunology. 2022; 12: 758525. https://doi.org/10.3389/fimmu.2021.758525

27. Llauradó-Calero E. et al. Eicosapentaenoic acid- and docosahexaenoic acid-rich fish oil in sow and piglet diets modifies blood oxylipins and immune indicators in both, sows and suckling piglets. Animal. 2022; 16(10): 100634. https://doi.org/10.1016/j.animal.2022.100634

28. Nguyen T.V., Yuan L., Azevedo M.S.P., Jeong K.-I., Gonzalez A.-M., Saif L.J. Transfer of maternal cytokines to suckling piglets: in vivo and in vitro models with implications for immunomodulation of neonatal immunity. Veterinary Immunology and Immunopathology. 2007; 117(3–4): 236–248. https://doi.org/10.1016/j.vetimm.2007.02.013

29. Quesnel H. Colostrum production by sows: variability of colostrum yield and immunoglobulin G concentrations. Animal. 2011; 5(10): 1546–1553. https://doi.org/10.1017/S175173111100070X

30. Toptygina A.P., Andreev Yu.Yu., Smerdova M.A., Zetkin A.Yu., Klykova T.G. Formation of humoral and cellular immunity to measles vaccine in adults. Russian Journal of Infection and Immunity. 2020; 10(1): 137–144 (in Russian). https://doi.org/10.15789/2220-7619-FOH-1334

31. Smirnova T.L., Portnova E.V., Sergeeva V.E. Immunity and pregnancy. Bulletin of the Chuvash University. 2009; 2: 79–85 (in Russian). https://elibrary.ru/kwhrnf

32. Kirgizov K.I., Skorobogatova E.V. Intravenous immunoglobulins: application of modern physiological solution is able to improve results of the therapy. Russian Journal of Pediatric Hematology and Oncology. 2015; 2(2): 77–83 (in Russian). https://elibrary.ru/twitlz

33. Than N.G., Hahn S., Rossi S.W., Szekeres-Bartho J. Editorial: Fetal-Maternal Immune Interactions in Pregnancy. Frontiers in Immunology. 2019; 10: 2729. https://doi.org/10.3389/fimmu.2019.02729

34. Alpatova N.A., Avdeeva Zh.I., Gayderova L.A., Lysikova S.L., Medunitsyn N.V. Immune Response Induced by Immunisation with Antiviral Vaccines. BIOpreparations. Prevention, Diagnosis, Treatment. 2020; 20(1): 21–29 (in Russian). https://doi.org/10.30895/2221-996X-2020-20-1-21-29

35. Brigadirov Yu.N., Kotsarev V.N., Shaposhnikov I.T., Lobanov A.E., Borisenko N.A. Metabolic status and post-vaccination immunity in cattle in the area of industrial emissions into the atmosphere. The Veterinarny Vrach. 2018; 2: 24–29 (in Russian). https://elibrary.ru/yvsqxs

36. Hultén E., Neil M., Einarsson S., Håkansson J. Energy Metabolism During Late Gestation and Lactation in Muciparous Sows in Relation to Backfat Thickness and the Interval from Weaning to First Oestrus. Acta Veterinaria Scandinavica. 1993; 34(1): 9–20. https://doi.org/10.1186/BF03548218

37. Afonina K.A., Ivanov V.A., Petrova S.A., Shamsutdinova E.M. Analysis of quantitative changes in peripheral blood leukocytes of experimental animals under the influence of copper-zinc-pyrite ore. Vestnik Bashkirskogo gosudarstvennogo meditsinskogo universiteta. 2018; S2–1: 1317–1321 (in Russian). https://elibrary.ru/yubfzz

38. Raimova A., Tursunova M.U., Saidov A.B. Comprehensive assessment of protein metabolism and iron metabolism in various categories of donors. Botkin readings. Collection of abstracts of the All-Russian Therapeutic Congress with international participation. St. Petersburg: Chelovek i yego zdorov′ye. 2023; 216–217 (in Russian). https://elibrary.ru/kbntfi

39. Hu L. et al. Metabolomic Profiling Reveals the Difference on Reproductive Performance between High and Low Lactational Weight Loss Sows. Metabolites. 2019; 9(12): 295. https://doi.org/10.3390/metabo9120295

40. Costermans N.G.J. et al. Influence of the metabolic state during lactation on milk production in modern sows. Animal. 2020; 14(12): 2543–2553. https://doi.org/10.1017/S1751731120001536

41. Kalhan S.C. Protein metabolism in pregnancy. The American Journal of Clinical Nutrition. 2000; 71(5): 1249S–1255S. https://doi.org/10.1093/ajcn/71.5.1249s

42. Skripkin V.S., Trukhachev V.I., Kvochko A.N., Agarkov A.V. The indicators of protein and nitrogen metabolism in pigs during pregnancy. Bulletin of KrasGAU. 2020; 12: 152–155 (in Russian). https://doi.org/10.36718/1819-4036-2020-12-152-155

43. Enthoven L.F. et al. The Effects of Pregnancy on Amino Acid Levels and Nitrogen Disposition. Metabolites. 2023; 13(2): 242. https://doi.org/10.3390/metabo13020242

44. Sereda T.I., Derkho M.A. The role of aminotrasferase activity in hen productivity. Agricultural Biology. 2014; 49(2): 72–77 (in Russian). https://elibrary.ru/sbjjrp

45. Provorov A.S., Lyubin N.A., Dezhatkina S.V., Provorova N.A., Gubeydullina Z.M. The lipid status of sows with use of water-soluble preparations beta-carotene. Vestnik of Ulyanovsk State Agricultural Academy. 2012; 4: 57–61 (in Russian). https://elibrary.ru/rkpfwp

46. Rosero D.S., Boyd R.D., Odle J., van Heugten E. Optimizing dietary lipid use to improve essential fatty acid status and reproductive performance of the modern lactating sow: a review. Journal of Animal Science and Biotechnology. 2016; 7: 34. https://doi.org/10.1186/s40104-016-0092-x

47. Wang W. et al. Effect of maternal dietary starch-to-fat ratio and daily energy intake during late pregnancy on the performance and lipid metabolism of primiparous sows and newborn piglets. Journal of Animal Science. 2022; 100(4): skac033. https://doi.org/10.1093/jas/skac033

48. Roland M.C.P., Lekva T., Godang K., Bollerslev J., Henriksen T. Changes in maternal blood glucose and lipid concentrations during pregnancy differ by maternal body mass index and are related to birthweight: A prospective, longitudinal study of healthy pregnancies. PLoS ONE. 2020; 15(6): e0232749. https://doi.org/10.1371/journal.pone.0232749