Infection of reindeer of the Yamalo-Nenets Autonomous Okrug with pathogens of vector-borne diseases

health, therefore, monitoring the prevalence of these diseases is an urgent area of scientific research.

Methods. The material for the research was stabilized reindeer blood (n = 83). Infection of animals with anaplasmosis, babesiosis, borreliosis, teileriosis and ehrlichiosis was established by PCR with subsequent separation of amplification products by vertical electrophoresis in polyacrylamide gel. The morphological profile of the blood was studied using the URIT-3020 analyzer.

Results. The genetic material of the pathogens of the genus Babesia was found in the blood of 18.1% of animals, with a maximum infection rate of adults (20.0%), and the DNA of tayleria was isolated in 2.4% of samples. Anaplasmosis infection averaged 3.6%, with a maximum infection rate of choruses (10.0%). The causative agent of ehrlichiosis was identified in 30.1% of the samples, while in 8.4% the carrier was in the form of monoinfection. Spirochaetosis was detected in 50.6% of the surveyed livestock, with a maximum infection of vazhenok (55.6%). In the form of monoinfection, borreliosis was found in 12.1% of reindeer. Coinfection of borreliosis and ehrlichiosis was most often observed (the average infection with the two pathogens was 19.3%). Mixinfection of Babesia, Borrelia and Ehrlichia was found in 10.8% of reindeer, and simultaneous carriage of Anaplasma, Babesia and Borrelia was detected in 1.2% of the studied livestock. Hematological studies have shown that in all age and gender groups, against the background of infection with vector-borne pathology, there is a decrease in the average volume of red blood cells (by 3.6–8.7%, р ≤ 0.05) and the hemoglobin content in the erythrocyte (by 3.4–4.8%, р ≤ 0.05).

1. Yuzhakov A.A. Ppersonal reindeer as the basis of preservation of reindeer husbandry. Scientific bulletin of Yamal-Nenets autonomous district. 2017; (4) 28–31 (in Russian). https://elibrary.ru/xnblbb

2. Fedorov V.I., Rumyanseva T.D., Robbek N.S., Sleptsov E.S., Vinokurov N.V. The question of the reindeer of Even breeds adaptation to the mountain taiga zone in the north-east of Russia. Genetics and breeding of animals. 2018; (1): 115–121 (in Russian). https://elibrary.ru/xsrfwx

3. Ilyina L.A. et al. Habitat as a determining factor for the reindeer rumen microbiome formation in Russian Arctic. Agricultural Biology. 2019; 54(6): 1177–1187. https://elibrary.ru/gdkxde

4. Kazanovsky E.S., Karabanov V.P., Klebenson K.A. Therapeutic and prophylactic efficacy of the composition of ganamectin and vaccine strain 55 against edemagenosis and anthrax of reindeer. Perm Agrarian Bulletin. 2014; (2): 60–65 (in Russian). https://elibrary.ru/sfbdgv

5. Zabrodin V.A. (ed.). Diseases of reindeer. St. Petersburg; Pushkin: North-West Centre of Interdisciplinary Researches of Problems of Food Maintenance. 2019; 222 (in Russian). ISBN 978-5-9905152-0-8 https://elibrary.ru/zziuzn

6. Kazanovsky E.S., Karabanov V.P., Klebenson K.A. Optimization of the annual treatment and preventive measures in the reindeer breeding. Veterinary medicine. 2019; (11): 9–11 (in Russian). https://elibrary.ru/gwnoxy

7. Kazanovsky E.S., Selyaninov Yu.O., Grekhova N.V., Karabanov V.P., Klebenson K.A. Improving the technology of combating dominant infections and parasitoses of reindeer. Problems of prevention and control of especially dangerous, exotic and poorly studied infectious diseases of animals. Proceedings of the International scientific and practical conference dedicated to the 50th anniversary of Federal Research Center for Virology and Microbiology. Pokrov: Federal Research Center for Virology and Microbiology. 2008; 1: 225–228 (in Russian). https://elibrary.ru/pvodqz

8. Laishev К.A., Zabrodin V.A., Prokudin A.V., Samandas A.M. Basic and poorly understood disease Taimyr population of wild reindeer. Genetics and breeding of animals. 2017; (1): 3–8 (in Russian). https://elibrary.ru/yornhj

9. Gavrilyeva L.Yu., Kokolova L.M. Northern piroplasmosis of domestic reindeer in Southern Yakutia. Veterinaria i kormlenie. 2022; (6): 21–24 (in Russian). https://elibrary.ru/zxyeey

10. Koryakina L.P. On cases of manifestation of blood parasitic disease in northern domestic deer in the territory of Yakutia. Vestnik ASAU. 2022; (4): 5–13 (in Russian). https://elibrary.ru/ojzabr

11. Nikolaev S.V., Romanenko T.M., Bessolitsyna E.A. Prevalence of vectorborne diseases among reindeer (Rangifer tarandus) Bolshezemelskaya and Malozemelskaya tundr [sic!]. Russian Journal of Parasitology. 2023; 17(4): 479–487 (in Russian). https://elibrary.ru/zhspry

12. Liberman E.L. Features of anaplasmosis and babesiosis of ruminants of the Tyumen region. PhD (Biology) Thesis. Tyumen. 2014. 174 (in Russian). https://elibrary.ru/nejpwb

13. Laroche M., Weeks E.N.I. Vector-borne bacterial diseases: a neglected field of infectious diseases research. Med Vet Entomol. 2023; 37(2): 177–178. DOI: 10.1111/mve.12646 Epub 2023 Feb 15 PMID: 36789732

14. Fanelli A. A historical review of Babesia spp. associated with deer in Europe: Babesia divergens / Babesia divergens-like, Babesia capreoli, Babesia venatorum, Babesia cf. odocoilei. Vet Parasitol. 2021; 294: 109433. DOI: 10.1016/j.vetpar.2021.109433

15. Nikolaev S.V., Romanenko T.M., Bessolitsyna E.A. Molecular diagnostics and prevalence of hemosporidiosis amond reindeer of the Nenets Autonomous Okrug. Veterinary medicine. 2022; (11): 35–38 (in Russian). https://elibrary.ru/elbpqt