Genotyping of the generic complex Caragana, growing in areas with an arid climate, using ISSR markers

Relevance. To increase the productivity and sustainability of artificial protective plantings, along with forestry activities, much attention is paid to the issues of genetic certification. Genotyping of the genus Caragana complex can currently only be carried out using nonspecific markers, since the genome of the studied objects growing in areas with an arid climate has not been sequenced. The purpose of the study was to genotype the Caragana genus complex using ISSR in an area with a characteristic arid climate.

Methods. To implement the planned program, field and laboratory experiments were carried out according to existing and newly developed methods.

Results. The study showed that loci UBC 880 (1100 bp) and UBC 818 (450 bp) may be markers of C. arborescens f. pyramidalis “Incomparable VNIALMI”, C. arborescens — UBC 823 (650 bp) and for C. frutex — UBC 815 (500 bp). The results obtained confirm the success of the new variety and its high resistance to drought. C. arborescens f. Pyramidalis “Incomparable VNIALMI” can be recommended for protective afforestation and agroforestry in arid areas.

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