Effect of interval between OPU sessions on the efficiency of oocyte retrieval at intensive using of donors of different cattle breeds
https://doi.org/10.32634/0869-8155-2025-393-04-95-100
Abstract
The article presents data on the efficiency of transvaginal ultrasound-guided oocyte retrieval (OPU) depending on the interval between sessions at intensive use of donors in cattle of various breeds. Donors of Kholmogory (n = 7), Istoben (n = 7) and Tagil (n = 4) cattle breeds were used in the experiment. Four OPU sessions were performed on each of the donors with each of two different intervals: 4 days and 3 days. The greatest number of follicles was found in heifers of the Kholmogor breed (13.23 ± 0.51 follicles versus 9.09 ± 0.44 and 9.38 ± 0.61 follicles, respectively, in heifers of the Istoben and first-lactation cows of Tagil breeds), while the interval between sessions did not significantly affect this indicator. In the first-lactation cows of the Tagil breed, a greatest number of cumulus-oocyte complexes (COCs) was obtained when using the interval between sessions of 3 days compared to 4 days — 5.69 ± 0.64 versus 4.06 ± 0.59 COCs (p < 0.05). There were no significant differences in the donors of the Kholmogor and Istoben breeds — 8.32 ± 0.75 versus 7.82 ± 0.59 COCs and 3.82 ± 0.40 versus 3.52 ± 0.57 COCs, respectively. The interval between sessions did not have a noticeable effect on the quality of the retrieved COCs. Thus, the absence of significant effect of different interval between OPU sessions on the number of obtained COCs, which are suitable for in vitro embryo production in all three studied breeds was shown (5.75 ± 0.54 vs 6.43 ± 0.66, 2.55 ± 0.45 vs 2.79 ± 0.48 and 2.88 ± 0.53 vs 4.00 ± 0.50 in donors of the Kholmogor, Istoben and Tagil breeds, respectively). This allows us to recommend conducting OPU sessions with alternating intervals of 4 days and 3 days for oocyte retrieval at intensive using of donors of above mentioned cattle breeds.
Supporting agencies: This research was funded by the Russian Science Foundation within (project No. 24-76-10028).
About the Authors
R. Yu. Chinarov
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Roman Yurievich Chinarov, Candidate of Veterinary Sciences, Senior Researcher at the Laboratory of Embryonic Technologies
60 Dubrovitsy settlement, Podolsk, Moscow region, 142132
V. A. Lukanina
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Viktoria Alexandrovna Lukanina, Candidate of Biological Sciences, Senior Researcher at the Laboratory of Embryonic Technologies
60 Dubrovitsy settlement, Podolsk, Moscow region, 142132
A. Yu. Dzhagaev
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Alan Yurievich Dzhagaev, Postgraduate Student, Junior Researcher at the Cryobiology Laboratory
60 Dubrovitsy settlement, Podolsk, Moscow region, 142132
G. N. Singina
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Galina Nikolaevna Singina, Candidate of Biological Sciences, Leading Researcher at the Laboratory of Embryonic Technologies
60 Dubrovitsy settlement, Podolsk, Moscow region, 142132
References
1. Nanda R. et al. Ovum Pick-up in Ruminants. Animal Reproduction Update. 2022; 1(1): 46-50.
2. Ferre L.B., Kjelland M.E., Strobech L.B., Hyttel P, Mermillod P, Ross P J. Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods. Animal. 2020; 14(5): 991-1004. https://doi.org/10.1017/S1751731119002775
3. Viana J.H.M. 2021 Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter. 2022; 40(4): 22-40.
4. Boler R., Kasimanickam R. Ovum pick up, in vitro embryo production, and embryo transfer as value-added theriogenology service in miniature Hereford cattle operation. Clinical Theriogenology. 2024; 16: 10528. https://doi.org/10.58292/CT.v16.10528
5. Boni R. Ovum pick-up in cattle: a 25 yr retrospective analysis. Animal Reproduction. 2012; 9(3): 362-369.
6. Nikitin G.S. Modern approaches for obtaining and cryoconservation of cattle embryos in vitro. International Journal of Veterinary Medicine. 2021; (3): 192-205 (in Russian). https://doi.org/10.17238/issn2072-2419.2021.3.192
7. Chinarov R.Yu. Developing the Ovum Pick-Up technology in cattle: state-of-the-art and research directions (review). Agricultural Biology. 2024; 59(2): 194-220. https://doi.org/10.15389/agrobiology.2024.2.194eng
8. Pestis V.K., Golubets L.V., Deshko A.S., Kyssa I.S., Popov M.V. Cow oocyte pick-up by the transvaginal puncture of follicles. Doklady of the National Academy of Sciences of Belarus. 2016; 60(1): 123-128 (in Russian). https://www.elibrary.ru/vspopl
9. Ferre L.B. et al. Transvaginal ultrasound-guided oocyte retrieval in cattle: State-of-the-art and its impact on the in vitro fertilization embryo production outcome. Reproduction in Domestic Animals. 2023; 58(3): 363-378. https://doi.org/10.1111/rda.14303
10. Chinarov R.Yu., Lukanina V.A., Singina G.N. Efficiency of Ovum Pick-Up in cows of Tagil breed. Veterinaria ikormlenie. 2024; (1): 89-94 (in Russian). https://doi.org/10.30917/ATT-VK-1814-9588-2024-1-19
11. Li F., Chen X., Pi W., Liu C., Shi Z. Collection of Oocytes Through Transvaginal Ovum Pick-up for In Vitro Embryo Production in Nanyang Yellow Cattle. Reproduction in Domestic Animals. 2007; 42(6): 666-670. https://doi.org/10.1111/j.1439-0531.2006.00842.x
12. Ali S., Lemma A., Degefa T., Jemal J. Effect of breed, parity and frequency of collection on quality and quantity of OPU derived oocytes. World news of natural sciences. 2021; 34: 88-97.
13. Ding L.-J. et al. Different intervals of ovum pick-up affect the competence of oocytes to support the preimplantation development of cloned bovine embryos. Molecular Reproduction and Development. 2008; 75(12): 1710-1715. https://doi.org/10.1002/mrd.20922
14. Golubets L.V. Efficiency of different modes of using donors in obtaining cattle embryos in vitro in the system of transvaginal aspiration of oocytes (TAO). Transactions of the educational establishment “Vitebsk the Order of ‘the Badge of Honor' State Academy of Veterinary Medicine". 2021; 57(2): 87-91 (in Russian). https://www.elibrary.ru/olfdxk
15. Chinarov R.Yu., Lukanina V.A., Singina G.N., Shedova E.N. Efficiency of oocytes retrieval during a repeated series of transvaginal ultrasound-guided puncture of follicles in Simmental heifers. Agrarian science. 2024; (9): 65-70 (in Russian). https://doi.org/10.32634/0869-8155-2024-386-9-65-70
Review
For citations:
Chinarov R.Yu., Lukanina V.A., Dzhagaev A.Yu., Singina G.N. Effect of interval between OPU sessions on the efficiency of oocyte retrieval at intensive using of donors of different cattle breeds. Agrarian science. 2025;1(4):95-100. (In Russ.) https://doi.org/10.32634/0869-8155-2025-393-04-95-100
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