Determination of the expression level of the LCORL gene in reindeer muscle tissue
https://doi.org/10.32634/0869-8155-2025-397-08-52-57
Abstract
As a result of the work, a comprehensive analysis of the connection of the transcriptional activity of the LCORL gene in the transverse breast muscle of the northern deer of the Nenetskaya breed with its killer mass was carried out.
A comparative analysis of the level of relative expression of the LCORL gene in the studied fabric in deer in various groups on the basis of “slaughter mass” showed that the LCORL gene is highly expressed in the slaughter group of 50.2–59.2 kg (Median = 0.367), and the lowest expression in the deer group 60.2–75.0 kg (Median = 0.036). The analysis of literary sources revealed that the tissue expression of the LCORL gene, taking into account the weight characteristics of the deer, was not previously studied. The results of this study provide new information about the features of the expression of the LCORL gene in the northern reindeer of the Nenetsk breed, taking into account tissue -specificity and letters.
An analysis of correlation ties using the Dersman criterion did not reveal a multidirectional dependence of the level of relative expression of the LCORL gene in samples of the transverse chest muscle with a slaughter mass of deer of different groups, which can apparently be due to the features of the sample of deer.
About the Authors
T. A. LarkinaRussian Federation
Tatyana Aleksandrovna Larkina, Candidate of Biological Sciences, Junior Researcher
55А Moscow highway, Pushkin, St. Petersburg, 196601
G. K. Peglivanyan
Russian Federation
Grigory Karapetovich Peglivanyan, Junior Researcher
55А Moscow highway, Pushkin, St. Petersburg, 196601
O. Yu. Barkova
Russian Federation
Olga Yurievna Barkova, Candidate of Biological Sciences, Senior Researcher
55А Moscow highway, Pushkin, St. Petersburg, 196601
A. V. Gabova
Russian Federation
Alina Valerievna Gabova, Laboratory Researcher, Master’s Degree
55А Moscow highway, Pushkin, St. Petersburg, 196601
N. V. Dementieva
Russian Federation
Natalia Viktorovna Dementieva, Candidate of Biological Sciences, Leading Researcher
55А Moscow highway, Pushkin, St. Petersburg, 196601
References
1. Yuzhakov A.A., Laishev K.A. Features of the growth and formation of the physique of nenets reindeer from birth to puberty. International Journal of Veterinary Medicine. 2022; (2): 104–111 (in Russian). https://doi.org/10.52419/issn2072-2419.2022.2.104
2. Mityukov A.S., Kaneva L.A., Zharikov Ya.A. Likely avenues of meat production in the northern regions of the Russian Federation. Izvestiya Saint-Petersburg State Agrarian University. 2015; 39: 129–131 (in Russian). https://www.elibrary.ru/uxwmcf
3. Loginov V.G. Reindeer husbandry as a basic branch of the North’s traditional agricultural sector. Agrarian Bulletin of the Urals. 2014; (11): 74–77 (in Russian). https://www.elibrary.ru/tajscb
4. Yuzhakov A.A., Romanenko T.M., Laishev K.A. Genogeographic variability reindeer Nenets breed. Izvestiya Saint-Petersburg State Agrarian University. 2017; 47: 115–122 (in Russian). https://www.elibrary.ru/zbezqx
5. McDevitt A.D. et al. Survival in the Rockies of an endangered hybrid swarm from diverged caribou (Rangifer tarandus) lineages. Molecular Ecology. 2009; 18(4): 665–679. https://doi.org/10.1111/j.1365-294X.2008.04050.x
6. Deniskova T.E. et al. Genetic characteristics of regional populations of Nenets reindeer breed (Rangifer tarandus). Agricultural Biology. 2018; 53(6): 1152–1161. https://doi.org/10.15389/agrobiology.2018.6.1152eng
7. Kharzinova V.R., Zinovieva N.A. Application of microsatellites in population genetic studies of reindeer (Rangifer tarandus) (review). Agricultural Science Euro-North-East. 2024; 25(4): 525–537 (in Russian). https://doi.org/10.30766/2072-9081.2024.25.4.525-537
8. Metzger J., Schrimpf R., Philipp U., Distl O. Expression Levels of LCORL Are Associated with Body Size in Horses. PLoS ONE. 2013; 8(2): e56497. https://doi.org/10.1371/journal.pone.0056497
9. Soranzo N. et al. Meta-Analysis of Genome-Wide Scans for Human Adult Stature Identifies Novel Loci and Associations with Measures of Skeletal Frame Size. PLoS Genetics. 2009; 5(4): e1000445. https://doi.org/10.1371/journal.pgen.1000445
10. Lindholm-Perry A.K. et al. Association, effects and validation of polymorphisms within the NCAPG-LCORL locus located on BTA6 with feed intake, gain, meat and carcass traits in beef cattle. BMC Genetics. 2011; 12: 103. https://doi.org/10.1186/1471-2156-12-103
11. Rubin C.-J. et al. Strong signatures of selection in the domestic pig genome. Proceedings of the National Academy of Sciences. 2012; 109(48): 19529–19536. https://doi.org/10.1073/pnas.1217149109
12. Al-Mamun H.A., Kwan P., Clark S.A., Ferdosi M.H., Tellam R., Gondro C. Genome-wide association study of body weight in Australian Merino sheep reveals an orthologous region on OAR6 to human and bovine genomic regions affecting height and weight. Genetics Selection Evolution. 2015; 47: 66. https://doi.org/10.1186/s12711-015-0142-4
13. Takasuga A. PLAG1 and NCAPG‐LCORL in livestock. Animal Science Journal. 2016; 87(2): 159–167. https://doi.org/10.1111/asj.12417
14. Lyu S., Arends D., Nassar M.K., Brockmann G.A. Fine mapping of a distal chromosome 4 QTL affecting growth and muscle mass in a chicken advanced intercross line. Animal Genetics. 2017; 48(3): 295–302. https://doi.org/10.1111/age.12532
15. Larkina T.A. et al. Evolutionary Subdivision of Domestic Chickens: Implications for Local Breeds as Assessed by Phenotype and Genotype in Comparison to Commercial and Fancy Breeds. Agriculture. 2021; 11(10): 914. https://doi.org/10.3390/agriculture11100914
16. Han Y.J., Chen Y., Liu Y., Liu X.L. Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China. Journal of Genetics. 2017; 96(1): 9–17. https://doi.org/10.1007/s12041-016-0732-0
17. Betsha M.W. et al. Sequencing of reindeer (Rangifer tarandus) genomes: insights into evolution, domestication and adaptation. Bioinformatics of genome regulation and structure/systems biology (BGRS/SB-2018). The 11th International conference. Novosibirsk: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences. 2018; 198. https://doi.org/10.18699/BGRSSB-2018-168
18. Weldenegodguad M. et al. Genome sequence and comparative analysis of reindeer (Rangifer tarandus) in northern Eurasia. Scientific Reports. 2020; 10: 8980. https://doi.org/10.1038/s41598-020-65487-y
19. Livak K.J., Schmittgen T.D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods. 2001; 25(4): 402–408. https://doi.org/10.1006/meth.2001.1262
20. Krutikova A.A., Barkova O.Yu. Analysis of the ligand-dependent nuclear receptor gene of corepressor type polymorphism in reindeer. International Journal of Veterinary Medicine. 2020; (4): 111–115 (in Russian). https://doi.org/10.17238/issn2072-2419.2020.4.111
Review
For citations:
Larkina T.A., Peglivanyan G.K., Barkova O.Yu., Gabova A.V., Dementieva N.V. Determination of the expression level of the LCORL gene in reindeer muscle tissue. Agrarian science. 2025;(8):52-57. (In Russ.) https://doi.org/10.32634/0869-8155-2025-397-08-52-57