Identification of polymorphic SNPs in IGF2BP2 and BMPR1B genes in Orenburg and Karachaev breeds of goats

Relevance. Reproductive traits of goats are of high economic value. IGF2BP2 and BMPR1B genes were proposed as candidate genes associated with reproductive traits of goats. However, their role in the formation of multiple pregnancy in goats is poorly understood. In this regard, a more detailed study of polymorphism in the IGF2BP2 and BMPR1B genes may deepen the knowledge of the genetic mechanisms underlying multiple pregnancy in goats, so we chose these genes as targets for the experiment.

Methods. Complete genomes of goats of Orenburg (n = 15) and Karachaev (n = 15) breeds were obtained by whole genome sequencing performed by NGS. From whole genomes we extracted the complete sequences of candidate genes affecting the reproductive abilities of goats (IGF2BP2 and BMPR1B), in which a search for single nucleotide polymorphisms (SNP) was performed. The most different SNPs in the analyzed genes were selected based on the calculation of F_ST values for each SNP within each gene, performed using PLINK 2 software.

Results. Comparison of the nucleotide sequence of the IGF2BP2 and BMPR1B genes in Karachaev and Orenburg goats, the most different SNPs were identified based on the calculation of F_ST values. The greatest differences in sequences were found in the BMPR1B gene: 11 SNPs with F_ST values from 0.513305 to 0.596852. While in the IGF2BP2 gene, only 2 SNPs were identified (F_ST = 0.453078).

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