The use of hexanucleotide SSR-markers for molecular genetic certification of Cannabis sativa L.
https://doi.org/10.32634/0869-8155-2025-398-09-99-105
Abstract
This research addresses the urgent issue of molecular genetic certification of hemp (Cannabis sativa L.) varieties, crucial amid rapid breeding progress and expanding industrial use. The aim of the research is a development a complex of SSR markers that will ensure reliable differentiation of hemp varieties for breeding and seed production practices and molecular genetic certification. The work presents the results of the analysis of twelve varieties of monoecious industrial hemp of the Central Russian ecotype using two sets of SSR markers: highly polymorphic dinucleotide markers and developed hexanucleotide microsatellite markers. The study included DNA extraction, PCR amplification, allele detection and statistical data processing. The results obtained showed an extremely high level of intravarietal polymorphism when using highly polymorphic markers, which complicates their use for genetic certification. The developed complex of 10 hexanucleotide SSR markers covering all chromosomes of the genome made it possible to carry out intravarietal differentiation of samples.
Keywords
Cannabis sativa L.,
molecular genetic certification,
SSR-markers,
genetic identification,
selection,
PCR,
dendrogram
Supporting agencies: Scientific research was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation on topic FGSS-2024-0002.
About the Authors
T. A. Bazanov
Federal Research Center for Bast Fiber Crops
Russian Federation
Russian Federation
Taras Aleksandrovich Bazanov, Candidate in Chemical Sciences, Leading researcher, head of the laboratory
17/56 Komsomolsky Аve., Tver, 170041
I. V. Ushapovsky
Federal Research Center for Bast Fiber Crops
Russian Federation
Russian Federation
Igor Valentinovich Ushapovsky, Candidate in Biological Sciences, Leading researcher, deputy director for science
17/56 Komsomolsky Аve., Tver, 170041
N. N. Loginova
Federal Research Center for Bast Fiber Crops
Russian Federation
Russian Federation
Natalya Nikolaevna Loginova, Researcher
17/56 Komsomolsky Аve., Tver, 170041
E. V. Minina
Federal Research Center for Bast Fiber Crops
Russian Federation
Russian Federation
Ekaterina Vitalievna Minina, Junior researcher
17/56 Komsomolsky Аve., Tver, 170041
P. D. Veresova
Federal Research Center for Bast Fiber Crops
Russian Federation
Russian Federation
Polina Dmitrievna Veresova, Junior researcher
17/56 Komsomolsky Аve., Tver, 170041
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Review
For citations:
Bazanov T.A., Ushapovsky I.V., Loginova N.N., Minina E.V., Veresova P.D. The use of hexanucleotide SSR-markers for molecular genetic certification of Cannabis sativa L. Agrarian science. 2025;(9):99-105. (In Russ.) https://doi.org/10.32634/0869-8155-2025-398-09-99-105
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