The effect of different concentrations of insulin-like growth factor-1 on in vitro maturation of oocytes and subsequent development of parthenogenetic embryos in cattle
https://doi.org/10.32634/0869-8155-2025-401-12-93-98
Abstract
Relevance. Insulin-like growth factor-1 (IGF-1) is involved in the regulation of meiosis in vivo and is therefore actively investigated as a potential regulator of oocyte quality aimed at improving the efficiency of in vitro embryo production biotechnologies. In the present study, we examined the effect of IGF-1 during bovine oocyte culture in terms of their in vitro maturation, embryonic development after artificial activation, and the quality of the resulting embryos.
Materials and methods. Oocytes isolated from ovarian follicles were cultured either in the absence (control) or presence of various concentrations of IGF-1 (5, 10, 20, 40, and 80 ng/ml). Matured oocytes were then artificially activated and cultured for 7 days to support embryonic development.
Results. IGF-1 had no effect on oocyte maturation, cleavage, or the number of nuclei in blastocyst. At the same time, a concentration-dependent effect of IGF-1 on the development of oocytes to the blastocyst stage was observed. In the control group, the blastocyst yield was 17.3±1.6%. The presence of IGF-1 in the maturation medium at concentrations of 5, 10, and 20 ng/ml increased this rate to 29.9±4.5, 30.7±3.6, and 29.6±2.3%, respectively (p < 0.05). Thus, at these concentrations IGF-1 can be recommended for in vitro maturation of bovine oocytes.
Keywords
Supporting agencies: The work was carried out with the financial support of the Russian Science Foundation (project No. 24-16-00261).
About the Authors
G. N. Singina
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Galina Nikolaevna Singina, Candidate of Biological Sciences, Head of the Laboratory of Embryonic Technologies
60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132
E. N. Shedova
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Ekaterina Nikolaevna Shedova, Research Associate
60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132
A. V. Lopukhov
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Alexandеr Viktorovich Lopukhov, Research Associate
60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132
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Review
For citations:
Singina G.N., Shedova E.N., Lopukhov A.V. The effect of different concentrations of insulin-like growth factor-1 on in vitro maturation of oocytes and subsequent development of parthenogenetic embryos in cattle. Agrarian science. 2025;(12):93-98. (In Russ.) https://doi.org/10.32634/0869-8155-2025-401-12-93-98
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