Prospects for the use of lactoferrin in animal husbandry
https://doi.org/10.32634/0869-8155-2026-403-02-40-59
Abstract
Relevance. Lactoferrin is a natural cationic iron-binding glycoprotein from the transferrin group. An important component of innate immunity, lactoferrin is a multifunctional protein with a broad spectrum of biological activity. Lactoferrin is widely used in pharmaceuticals and has potential application in animal husbandry.
Methods. The search for potentially relevant articles was carried out by keywords in electronic databases and open Internet access.
Results. A literature review revealed the antimicrobial, antifungal, antiviral, and immunomodulatory activity of lactoferrin. Lactoferrin is increasingly used in animal husbandry and veterinary medicine; however, the complexity of its production and purification hinders this process. The development and introduction of lactoferrin-based dietary supplements aimed at immunocorrection and strengthening the body of farm animals is promising. Lactoferrin can be used as a substance for the creation of a therapeutic agent in veterinary medicine. In addition, extensive opportunities could be created for the industry by creating herds of transgenic animals producing a biosimilar to human lactoferrin.
Supporting agencies: This scientific review was prepared as part of the research carried out under the state assignment for scientific research work No. FGUS-2024-0002 of the Gorbatov Federal Scientific Center for Food Systems.
About the Authors
Ya. M. Rebezov
Yaroslav-the-Wise Novgorod State University
Russian Federation
Russian Federation
Yaroslav Maksimovich Rebezov, Candidate of Biological Sciences, Researcher at the Applied Biotechnology Sector of the Educational and Scientific Research Laboratory of the Institute of Chemical Technology
41 Bolshaya Sankt-Peterburgskaya St., Veliky Novgorod,
173003
M. B. Rebezov
Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Maksim Borisovich Rebezov, Doctor of Agricultural Sciences, Professor, Chief Researcher
26 Talalikhin St., Moscow, 109316
U. Yu. Medvedeva
Yaroslav-the-Wise Novgorod State University
Russian Federation
Russian Federation
Ulyana Yurievna Medvedeva, Candidate of Agricultural Sciences, Associate Professor of the Department of Technology of Production and Processing of Agricultural Products
41 Bolshaya Sankt-Peterburgskaya St., Veliky Novgorod,
173003
at the Institute of Chemical Technology
References
1. Zhilyakova E.T. et al. Properties and Prospects of Application of the Whey Protein Lactoferrin in Medicine and Veterinary Medicine (Review). Drug development & registration. 2022; 11(1): 32–39 (in Russian). https://doi.org/10.33380/2305-2066-2022-11-1-32-39
2. Fisinin V. Increasing meat and egg production. Animal Husbandry of Russia. 2023; (1): 12–14 (in Russian). EDN BKNRDW
3. Gonova O.V., Malygin A.A., Lukina V.A., Vorobyova O.K. Modernization of dairy and meat cattle breeding in agricultural formations: innovative approach. Modern high technologies. Regional application. 2021; (4): 86–92 (in Russian). EDN GQQZLO
4. Yarkova T.M. The status and problems of dairy cattle breeding in Russia. Food Policy and Security. 2024; 11(1): 119–134 (in Russian). EDN OJJAQK
5. Sadovskaya T.N. Lactoferrin, its properties, production and application in zootechnics and veterinary medicine. Fundamental and applied science: topical issues of theory and practice. Collection of articles from the IV International scientific and practical conference. Penza: Nauka i Prosveshcheniye. 2023; 66–70 (in Russian). EDN SQQLXX
6. Ashraf M.F. et al. Nutraceutical and Health-Promoting Potential of Lactoferrin, an Iron-Binding Protein in Human and Animal: Current Knowledge. Biological Trace Element Research. 2024; 202(1): 56–72. https://doi.org/10.1007/s12011-023-03658-4
7. Demir R., Sarıtaş S., Bechelany M., Karav S. Lactoferrin: Properties and Potential Uses in the Food Industry. International Journal of Molecular Sciences. 2025; 26(4): 1404. https://doi.org/10.3390/ijms26041404
8. Gudok A.A., Deykin A.V. Lactoferrin — prospects for use in the food, pharmaceutical and agricultural industries. Collection of scientific papers of the All-Russian Research Institute of Sheep and Goat Breeding. 2016; 9(1): 421–424 (in Russian). EDN WVJGTH
9. Pierce A. et al. Molecular cloning and sequence analysis of bovine lactotransferrin. European Journal of Biochemistry. 1991; 196(1): 177–184. https://doi.org/10.1111/j.1432-1033.1991.tb15801.x
10. Wang B., Timilsena Y.P., Blanch E., Adhikari B. Lactoferrin: Structure, function, denaturation and digestion. Critical Reviews in Food Science and Nutrition. 2019; 59(4): 580–596. https://doi.org/10.1080/10408398.2017.1381583
11. Trubitsina T.P. et al. Problems and prospects of using recombinant human lactoferrin and its derivatives. Problems of productive animal biology. 2018; (4): 5–26 (in Russian). https://doi.org/10.25687/1996-6733.prodanimbiol.2018.3.5-26
12. Zorina V.N. Pattern of lactoferrin anti-influenza virus inhibitory activity. Russian Journal of Infection and Immunity. 2020; 10(1): 49–54 (in Russian). https://doi.org/10.15789/2220-7619-POL-1156
13. Arcella A. et al. In vitro and in vivo effect of human lactoferrin on glioblastoma growth. Journal of Neurosurgery. 2015; 123(4): 1026–1035. https://doi.org/10.3171/2014.12.jns14512
14. Baker E.N., Baker H.M. A structural framework for understanding the multifunctional character of lactoferrin. Biochimie. 2009; 91(1): 3–10. https://doi.org/10.1016/j.biochi.2008.05.006
15. Baker H.M., Baker E.N. Lactoferrin and Iron: structural and dynamic aspects of binding and release. BioMetals. 2004; 17(3): 209–216. https://doi.org/10.1023/B:BIOM.0000027694.40260.70
16. Lönnerdal B. Nutritional and physiologic significance of human milk proteins. The American Journal of Clinical Nutrition. 2003; 77(6): 1537S–1543S. https://doi.org/10.1093/ajcn/77.6.1537S
17. Pan Y., Rowney M., Guo P., Hobman P. Biological properties of lactoferrin: an overview. Australian Journal of Dairy Technology. 2007; 62(1): 31–42.
18. Siqueiros-Cendón T., Arévalo-Gallegos S., Iglesias-Figueroa B.F., García-Montoya I.A., Salazar-Martínez J., Rascón-Cruz Q. Immunomodulatory effects of lactoferrin. Acta Pharmacologica Sinica. 2014; 35(5): 557–566. https://doi.org/10.1038/aps.2013.200
19. Czosnykowska-Łukacka M., Orczyk-Pawiłowicz M., Broers B., Królak-Olejnik B. Lactoferrin in Human Milk of Prolonged Lactation. Nutrients. 2019; 11(10): 2350. https://doi.org/10.3390/nu11102350
20. Sánchez L., Aranda P., Pérez M., Calvo M. Concentration of Lactoferrin and Transferrin throughout Lactation in Cow’s Colostrum and Milk. Biological Chemistry. 1988; 369(2): 1005–1008. https://doi.org/10.1515/bchm3.1988.369.2.1005
21. Hiss S., Meyer T., Sauerwein H. Lactoferrin concentrations in goat milk throughout lactation. Small Ruminant Research. 2008; 80(1–3): 87–90. https://doi.org/10.1016/j.smallrumres.2008.07.027
22. Conesa C. et al. Isolation of lactoferrin from milk of different species: Calorimetric and antimicrobial studies. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2008; 150(1): 131–139. https://doi.org/10.1016/j.cbpb.2008.02.005
23. Konuspayeva G., Serikbayeva A., Loiseau G., Narmuratova M., Faye B. Lactoferrin of camel milk of Kazakhstan. Faye B., Esenov P. (eds.). Desertification Combat and Food Safety: The Added Value of Camel Producers. IOS Press. 2005; 158–167.
24. Montagne P., Cuilliere M., Mole C., Bene M., Faure G. Changes in lactoferrin and lysozyme levels in human milk during the first twelve weeks of lactation. Bioactive compounds in human milk. 2001; 501: 241–247. https://doi.org/10.1007/978-1-4615-1371-1_30
25. Kehoe S.I., Jayarao B.M., Heinrichs A.J. A survey of bovine colostrum composition and colostrum management practices on Pennsylvania dairy farms. Journal of Dairy Science. 2007; 90(9): 4108–4116. https://doi.org/10.3168/jds.2007-0040
26. Konuspayeva G., Faye B., Loiseau G., Levieux D. Lactoferrin and immunoglobulin contents in camel’s milk (Camelus bactrianus, Camelus dromedarius, and Hybrids) from Kazakhstan. Journal of Dairy Science. 2007; 90(1): 38–46. https://doi.org/10.3168/jds.S0022-0302(07)72606-1
27. Goldman I.L., Deikin A.V., Sadchikova E.R. Human Lactoferrin Can Be Alternative to Antibiotics. Proceedings of the World medical conference. WSEAS Press. 2010; 27–38. EDN FIAXCE
28. Kanyshkova T.G., Buneva V.N., Nevinsky G.A. Lactoferrin and Its Biological Functions. Biochemistry (Moscow). 2001; 66(1): 1–7. https://doi.org/10.1023/A:1002817226110
29. Fomina U.O., Tutova O.A. Study of antibacterial properties lactoferrin and the possibilities of its use in veterinary medicine. Youth science — for the development of the agro-industrial complex. Proceedings of the IV International scientific and practical conference of students, graduate students, and young scientists. Kursk: Kursk State Agrarian University. 2024; 2: 415–420 (in Russian). EDN AHQUDT
30. Borisov N. Whole milk replacers for calves: to use or not?. Effektivnoye zhivotnovodstvo. 2021; (2): 79–85 (in Russian). EDN CJDLRL
31. Lukashevich V.S. et al. Production of recombinant human lactoferrin from the milk of goat-producers and its physiological effects. Doklady of the National Academy of Sciences of Belarus. 2016; 60(1): 72–81 (in Russian). EDN VSPOMT
32. Metleva A.S., Vatsueva N.N. Lactoferrin: problems and prospects of use in veterinary medicine (review). Relevant scientific and technical means and agricultural problems. Proceedings of the IX National scientific and practical conference with international participation. Kemerovo: Kuzbass State Agricultural Academy. 2022; 686–689 (in Russian). EDN KFLGZC
33. Gudok A.A., Deykin A.V. Lactoferrin: use prospects and analysis of actual results. Russian Scientist. 2017; 1(1): 3–12 (in Russian). EDN PAYMRB
34. Bukharin O.V., Valyshev A.V., Valysheva I.V. The role of lactoferrin in anti-infectious defense. Advances in Current Biology. 2011; 131(2): 135–144 (in Russian). EDN NTRVJF
35. Chen P.-W., Jheng T.T., Shyu C.-L., Mao F.C. Antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. Journal of Dairy Science. 2013; 96(3): 1438–1446. https://doi.org/10.3168/jds.2012-6112
36. Zorina V.N., Vorobyova O.N., Zorin N.A. Antimicrobial activity of the human and bovine lactoferrin against gram-positive bacteria and Candida albicans. Journal of Microbiology, Epidemiology and Immunobiology. 2018; 95(2): 54–58 (in Russian). https://doi.org/10.36233/0372-9311-2018-2-54-58
37. Jenssen H., Hancock R.E.W. Antimicrobial properties of lactoferrin. Biochimie. 2009; 91(1): 19–29. https://doi.org/10.1016/j.biochi.2008.05.015
38. Anand N., Kanwar R.K, Sehgal R., Kanwar J.R. Antiparasitic and Immunomodulatory Potential of Oral Nanocapsules Encapsulated Lactoferrin Protein Against Plasmodium Berghei. Nanomedicine. 2016; 11(1): 47–62. https://doi.org/10.2217/nnm.15.181
39. Yen C.-C. et al. Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection. BioMetals. 2011; 24(4): 585–594. https://doi.org/10.1007/s10534-011-9423-8
40. Legrand D., Pierce A., Elass E., Carpentier M., Mariller C., Mazurier J. Lactoferrin Structure and Functions. Bösze Z. (ed.). Bioactive Components of Milk. New York, NY: Springer. 2008; 163–194. https://doi.org/10.1007/978-0-387-74087-4_6
41. Zarzosa-Moreno D. et al. Lactoferrin and Its Derived Peptides: An Alternative for Combating Virulence Mechanisms Developed by Pathogens. Molecules. 2020; 25(24): 5763. https://doi.org/10.3390/molecules25245763
42. Kot A.N., Petrushko E.V., Budevich A.I., Prilovskaya E.I., Mikhailova O.I., Ubushaev B.S. Physiological state and productivity of calves fed goat milk containing recombinant human lactoferrin. Zootechnical Science of Belarus. 2022; 57(1): 235–243 (in Russian). https://doi.org/10.47612/0134-9732-2022-57-1-235-243
43. Wróbel M., Małaczewska J., Kaczorek-Łukowska E. Antiviral Effect of Bovine Lactoferrin against Enterovirus E. Molecules. 2022; 27(17): 5569. https://doi.org/10.3390/molecules27175569
44. Titov E.I., Tikhomirova N.A., Ionova I.I. Investigation of the ironbinding capacity of the bovine lactoferrin. Problems of nutrition. 2019; 88(1): 91–96 (in Russian). https://doi.org/10.24411/0042-8833-2019-10011
45. Puddu P., Borghi P., Gessani S., Valenti P., Belardelli F., Seganti L. Antiviral effect of bovine lactoferrin saturated with metal ions on early steps of human immunodeficiency virus type 1 infection. The International Journal of Biochemistry & Cell Biology. 1998; 30(9): 1055– 1063. https://doi.org/10.1016/S1357-2725(98)00066-1
46. Małaczewska J., Kaczorek-Łukowska E., Wójcik R., Siwicki A.K. Antiviral effects of nisin, lysozyme, lactoferrin and their mixtures against bovine viral diarrhoea virus. BMC Veterinary Research. 2019; 15: 318. https://doi.org/10.1186/s12917-019-2067-6
47. Metleva A.S. The effect of lactoferrin on hematological parameters of blood and intestinal microflora of calves with diarrhea. Innovative solutions in the agro-industrial complex. 2024; (2): 63–73 (in Russian). EDN VNFGIK
48. Habing G., Harris K., Schuenemann G.M., Piñeiro J.M., Lakritz J., Clavijo X.A. Lactoferrin reduces mortality in preweaned calves with diarrhea. Journal of Dairy Science. 2017; 100(5): 3940–3948. https://doi.org/10.3168/jds.2016-11969
49. Pempek J.A., Watkins L.R., Bruner C.E., Habing G.G. A multisite, randomized field trial to evaluate the influence of lactoferrin on the morbidity and mortality of dairy calves with diarrhea. Journal of Dairy Science. 2019; 102(10): 9259–9267. https://doi.org/10.3168/jds.2019-16476
50. Jahani S., Shakiba A., Jahani L. The Antimicrobial Effect of Lactoferrin on Gram-Negative and Gram-Positive Bacteria. International Journal of Infection. 2015; 2(3): e27954. https://doi.org/10.17795/iji27594
51. Radchikov V.F., Kot A.N., Petruschko E.V., Prilovskaya E.I. The productive effect of lactoferrin in calves’ diets. Actual problems of veterinary science and intensive animal husbandry. Collected papers of the IV International scientific and practical conference. Bryansk: Bryansk State Agrarian University. 2025; 1: 191–195 (in Russian). EDN YDDFZN
52. Bogdanovich D.M., Petruschko E.V. Expression of recombinant human lactoferrin in the milk of goat-producers during the year. Novosti nauki v APK. 2018; (2): 168–171 (in Russian). EDN UISYDA
53. Lu J. et al. Antibacterial and Anti-biofilm Activity of the Human Breast Milk Glycoprotein Lactoferrin against Group B Streptococcus. ChemBioChem. 2021; 22(12): 2124–2133. https://doi.org/10.1002/cbic.202100016
54. Ochoa T.J., Cleary T.G. Effect of lactoferrin on enteric pathogens. Biochimie. 2009; 91(1): 30–34. https://doi.org/10.1016/j.biochi.2008.04.006
55. Gomez H.F., Ochoa T.J., Carlin L.G., Cleary T.G. Human lactoferrin impairs virulence of Shigella flexneri. The Journal of Infectious Diseases. 2003; 187(1): 87–95. https://doi.org/10.1086/345875
56. Massucci M.T. et al. Proteolytic activity of bovine lactoferrin. BioMetals. 2004; 17(3): 249–255. https://doi.org/10.1023/b:biom.0000027700.90780.45
57. Ongena R., Dierick M., Vanrompay D., Cox E., Devriendt B. Lactoferrin impairs pathogen virulence through its proteolytic activity. Frontiers in Veterinary Science. 2024; 11: 1428156. https://doi.org/10.3389/fvets.2024.1428156
58. García-Montoya I.A., Siqueiros Cendón T., Arévalo-Gallegos S., Rascón-Cruz Q. Lactoferrin a multiple bioactive protein: An overview. Biochimica et Biophysica Acta (BBA) — General Subjects. 2012; 1820(3): 226–236. https://doi.org/10.1016/j.bbagen.2011.06.018
59. Valenti P., Antonini G. Lactoferrin. Cellular and Molecular Life Sciences. 2005; 62(22): 2576–2587. https://doi.org/10.1007/s00018-005-5372-0
60. Dierick M, Van der Weken H., Rybarczyk J., Vanrompay D., Devriendt B., Cox E. Porcine and Bovine Forms of Lactoferrin Inhibit Growth of Porcine Enterotoxigenic Escherichia coli and Degrade Its Virulence Factors. Applied and Environmental Microbiology. 2020; 86(24): e00524-20. https://doi.org/10.1128/AEM.00524-20
61. Bhimani R.S., Vendrov Y., Furmanski P. Influence of lactoferrin feeding and injection against systemic staphylococcal infections in mice. Journal of Applied Microbiology. 1999; 86(1): 135–144. https://doi.org/10.1046/j.1365-2672.1999.00644.x
62. Lee H.-Y. et al. Potential antimicrobial effects of human lactoferrin against oral infection with Listeria monocytogenes in mice. Journal of Medical Microbiology. 2005; 54(11): 1049–1054. https://doi.org/10.1099/jmm.0.45918-0
63. Kutila T., Pyörälä S., Saloniemi H., Kaartinen L. Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens. Acta Veterinaria Scandinavica. 2003; 44(1): 35–42. https://doi.org/10.1186/1751-0147-44-35
64. González-Chávez S.A., Arévalo-Gallegos S., Rascón-Cruz Q. Lactoferrin: structure, function and applications. International Journal of Antimicrobial Agents. 2009; 33(4): 301.e1–301.e8. https://doi.org/10.1016/j.ijantimicag.2008.07.020
65. Luna-Castro S., Aguilar-Romero F., Samaniego-Barrón L., Godínez-Vargas D., de la Garza M. Effect of bovine apo-lactoferrin on the growth and virulence of Actinobacillus pleuropneumoniae. BioMetals. 2014; 27(5): 891–903. https://doi.org/10.1007/s10534-014-9752-5
66. Samokhina L.S., Ganina V.I., Ionova I.I., Komolova G.S., Golovin M.A. Bifidogenic properties of lactoferrin peptides of cow milk. Problems of Biological, Medical and Pharmaceutical Chemistry. 2016; 19(2): 49–53 (in Russian). EDN VRXWBP
67. Bogdanovich D.M., Prilovskaya E.I. The use of lactoferrin in feeding calves. Agricultural science in the context of modernization and digital development of the Russian agro-industrial complex. Collection of articles based on the materials of the International scientific and practical conference. Kurgan: Kurgan State Agricultural Academy. 2022; 82–85 (in Russian). EDN BKXIKX
68. Xu G. et al. Lactoferrin-derived peptides and Lactoferricin chimera inhibit virulence factor production and biofilm formation in Pseudomonas aeruginosa. Journal of Applied Microbiology. 2010; 109(4): 1311–1318. https://doi.org/10.1111/j.1365-2672.2010.04751.x
69. Paredes J.L., Sparks H., White A.C.Jr., Martinez-Traverso G., Ochoa T., Castellanos-González A. Killing of Cryptosporidium sporozoites by Lactoferrin. The American Journal of Tropical Medicine and Hygiene. 2017; 97(3): 774–776. https://doi.org/10.4269/ajtmh.16-0804
70. Berlutti F. et al. Both lactoferrin and iron influence aggregation and biofilm formation in Streptococcus mutans. BioMetals. 2004; 17(3): 271–278. https://doi.org/10.1023/B:BIOM.0000027704.53859.d3
71. Bogdanovich D.M., Brovko T.N., Shevtsov I.N., Glivanskaya O.I., Grodnikova N.A. Effect of recombinant human lactoferrin on biological full value and sanitary quality of boars’ semen. Zootechnical Science of Belarus. 2018; 53(1): 21–28 (in Russian). EDN YNDKPB
72. Velusamy S.K., Fine D.H., Velliyagounder K. Prophylactic effect of human lactoferrin against Streptococcus mutans bacteremia in lactoferrin knockout mice. Microbes and Infection. 2014; 16(9): 762–767. https://doi.org/10.1016/j.micinf.2014.07.009
73. Hwang S.-A., Kruzel M.L., Actor J.K. Immunomodulatory effects of recombinant lactoferrin during MRSA infection. International Immunopharmacology. 2014; 20(1): 157–163. https://doi.org/10.1016/j.intimp.2014.02.029
74. Gomes F., Henriques M. Control of Bovine Mastitis: Old and Recent Therapeutic Approaches. Current Microbiology. 2016; 72(4): 377–382. https://doi.org/10.1007/s00284-015-0958-8
75. Shimazaki K.-i., Kawai K. Advances in lactoferrin research concerning bovine mastitis. Biochemistry and Cell Biology. 2017; 95(1): 69–75. https://doi.org/10.1139/bcb-2016-0044
76. Simojoki H., Hyvönen P., Orro T., Pyörälä S. High concentration of human lactoferrin in milk of rhLf-transgenic cows relieves signs of bovine experimental Staphylococcus chromogenes intramammary infection. Veterinary Immunology and Immunopathology. 2010; 136(3–): 265–271. https://doi.org/10.1016/j.vetimm.2010.03.017
77. Barrington K.J., Assaad M.-A., Janvier A. The Lacuna Trial: a double-blind randomized controlled pilot trial of lactoferrin supplementation in the very preterm infant. Journal of Perinatology. 2016; 36(8): 666–669. https://doi.org/10.1038/jp.2016.24
78. Kieckens E., Rybarczyk J., Cox E., Vanrompay D. Antibacterial and immunomodulatory activities of bovine lactoferrin against Escherichia coli O157:H7 infections in cattle. BioMetals. 2018; 31(3): 321–330. https://doi.org/10.1007/s10534-018-0082-x
79. Tanhaiean A., Azghandi M., Razmyar J., Mohammadi E., Sekhavati M.H. Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens. Microbial Pathogenesis. 2018; 122: 73–78. https://doi.org/10.1016/j.micpath.2018.06.012
80. Giansanti F., Panella G., Leboffe L., Antonini G. Lactoferrin from milk: Nutraceutical and Pharmacological Properties. Pharmaceuticals. 2016; 9(4): 61. https://doi.org/10.3390/ph9040061
81. Frontera L.S., Moyano S., Quassollo G., Lanfredi-Rangel A., Rópolo A.S., Touz M.C. Lactoferrin and lactoferricin endocytosis halt Giardia cell growth and prevent infective cyst production. Scientific Reports. 2018; 8: 18020. https://doi.org/10.1038/s41598-018-36563-1
82. Sinnis P., Willnow T.E., Briones M.R., Herz J., Nussenzweig V. Remnant lipoproteins inhibit malaria sporozoite invasion of hepatocytes. The Journal of Experimental Medicine. 1996; 184(3): 945–954. https://doi.org/10.1084/jem.184.3.945
83. Abd El Monsef et al. Effects of prebiotic (lactoferrin) and diclazuril on broiler chickens experimentally infected with Eimeria tenella. Frontiers in Veterinary Science. 2024; 11: 1416459. https://doi.org/10.3389/fvets.2024.1416459
84. Fernandes K.E., Carter D.A. The Antifungal Activity of Lactoferrin and Its Derived Peptides: Mechanisms of Action and Synergy with Drugs against Fungal Pathogens. Frontiers in Microbiology. 2017; 8: 2. https://doi.org/10.3389/fmicb.2017.00002
85. Pang C.N.I., Lai Y.-W., Campbell L.T., Chen S.C.-A., Carter D.A., Wilkins M.R. Transcriptome and network analyses in Saccharomyces cerevisiae reveal that amphotericin B and lactoferrin synergy disrupt metal homeostasis and stress response. Scientific Reports. 2017; 7: 40232. https://doi.org/10.1038/srep40232
86. Fernandes K.E., Weeks K., Carter D.A. Lactoferrin Is Broadly Active against Yeasts and Highly Synergistic with Amphotericin B. Antimicrobial Agents and Chemotherapy. 2020; 64(5): e02284-19. https://doi.org/10.1128/aac.02284-19
87. Viejo-Díaz M., Andrés M.T., Fierro J.F. Modulation of In Vitro Fungicidal Activity of Human Lactoferrin against Candida albicans by Extracellular Cation Concentration and Target Cell Metabolic Activity. Antimicrobial Agents and Chemotherapy. 2004; 48(4): 1242–1248. https://doi.org/10.1128/aac.48.4.1242-1248.2004
88. Brouwer C. et al. Synthetic Human Lactoferrin Peptide hLF(1–11) Shows Antifungal Activity and Synergism with Fluconazole and Anidulafungin Towards Candida albicans and Various Non-Albicans Candida Species, Including Candidozyma auris. Antibiotics. 2025; 14(7): 671. https://doi.org/10.3390/antibiotics14070671
89. Sadchikov P.E., Goldman I.L., Razin S.V., Chernousov A.D., Alekseeva L.I., Sadchikova E.R. The molecular mechanism of lactoferrin influence on bone formation. Osteoporosis and Bone Diseases. 2016; 19(3): 12–22 (in Russian). EDN YGDFNN
90. Li Q. et al. Effects of Recombinant Human Lactoferrin on Osteoblast Growth and Bone Status in Piglets. Animal Biotechnology. 2018; 29(2): 90–99. https://doi.org/10.1080/10495398.2017.1313269
91. Guo H.Y. et al. Orally Administered Lactoferrin Preserves Bone Mass and Microarchitecture in Ovariectomized Rats. The Journal of Nutrition. 2009; 139(5): 958–964. https://doi.org/10.3945/jn.108.100586
92. Saki A.A., Mahmoudi H. Effects of in ovo injection of bovine lactoferrin before incubation in layer breeder eggs on tibia measurements and performance of laying hens. Animal. 2015; 9(11): 1813–1819. https://doi.org/10.1017/S1751731115001093
93. Klasing K.C., Humphrey B.D., Huang N. Rice Expressing Lactoferrin and Lysozyme Has Antibiotic-Like Properties When Fed to Chicks. The Journal of Nutrition. 2002; 132(6): 1214–1218. https://doi.org/10.1093/jn/132.6.1214
94. Lee S.H., de Boer H.A. Production of biomedical proteins in the milk of transgenic dairy cows: the state of the art. Journal of Controlled Release. 1994; 29(3): 213–221. https://doi.org/10.1016/0168-3659(94)90068-X
95. Agievitch I.S., Kastsianevich A.A., Falkouskaya U.V., Birukou R.M. World practice for production of recombinant human lactoferrin (review). Microbial biotechnology: fundamental and applied aspects. Collection of scientific papers. Minsk: Belorusskaya nauka. 2017; 9: 9–30 (in Russian). EDN KZXMIQ
96. Brock J.H. The physiology of lactoferrin. Biochemistry and Cell Biology. 2002; 80(1): 1–6. https://doi.org/10.1139/o01-212
97. Legrand D. Overview of Lactoferrin as a Natural Immune Modulator. The Journal of Pediatrics. 2016; 173(S): S10–S15. https://doi.org/10.1016/j.jpeds.2016.02.071
98. Yang D., de la Rosa G., Tewary P., Oppenheim J.J. Alarmins link neutrophils and dendritic cells. Trends in Immunology. 2009; 30(11): 531–537. https://doi.org/10.1016/j.it.2009.07.004
99. Dhennin-Duthille I., Masson M., Damiens E., Fillebeen C., Spik G., Mazurier J. Lactoferrin upregulates the expression of CD4 antigen through the stimulation of the mitogen-activated protein kinase in the human lymphoblastic T Jurkat cell line. Journal of Cellular Biochemistry. 2000; 79(4): 583–593.
100. Iglesias-Figueroa B.F., Espinoza-Sánchez E.A., SiqueirosCendón T.S., Rascón-Cruz Q. Lactoferrin as a nutraceutical protein from milk, an overview. International Dairy Journal. 2019; 89: 37–41. https://doi.org/10.1016/j.idairyj.2018.09.004
101. Prilovskaya E.I. Defrosted milk of goats-producers of recombinant lactoferrin in the diet of calves aged 1–30 days. Actual problems of intensive development of animal husbandry. Collection of scientific articles. Gorki: Belarusian State Agricultural Academy. 2023; 26(1): 136–144 (in Russian). EDN SIIYED
102. Joslin R.S., Erickson P.S., Santoro H.M., Whitehouse N.L., Schwab C.G., Rejman J.J. Lactoferrin supplementation to dairy calves. Journal of Dairy Science. 2002; 85(5): 1237–1242. https://doi.org/10.3168/jds.S0022-0302(02)74187-8
103. Ma L., Zhu Y., Zhu La A.L.T., Lourenco J.M., Callaway T.R., Bu D. Schizochytrium sp. and lactoferrin supplementation alleviates Escherichia coli K99-induced diarrhea in preweaning dairy calves. Journal of Dairy Science. 2024; 107(3): 1603–1619. https://doi.org/10.3168/jds.2023-23466
104. Omar N.A., Abdel-Aziz A.M., Wafa W.M., El-Nagar H.A. Effect of lactoferrin supplementation on blood profile, immunity and growth performance in newly born calves. Journal of the Egyptian Veterinary Medical Association. 2022; 22: 229–245.
105. Prgomet C., Prenner M.L., Schwarz F.J., Pfaffl M.W. Effect of lactoferrin on selected immune system parameters and the gastrointestinal morphology in growing calves. Journal of Animal Physiology and Animal Nutrition. 2007; 91(3–4): 109–119. https://doi.org/10.1111/j.1439-0396.2006.00649.x
106. Cowles K.E., White R.A., Whitehouse N.L., Erickson P.S. Growth Characteristics of Calves Fed an Intensified Milk Replacer Regimen with Additional Lactoferrin. Journal of Dairy Science. 2006; 89(12): 4835–4845. https://doi.org/10.3168/jds.S0022-0302(06)72532-2
107. Mallaki M., Hosseinkhani A., Taghizadeh A., Paya H., Hamidian Gh. Effect of Lactoferrin and Probiotic on Health Status and Reduction of Escherichia Coli Infection in Ghezel Lambs in Preweaning Phase. Veterinary Research and Biological Products. 2021; 34(1): 55–62. https://doi.org/10.22092/vj.2020.128115.1634
108. El-Ashker M., Risha E., Abdelhamid F., Ateya A. Potential immune modulating properties and antioxidant activity of supplementing commercially available lactoferrin and/or Lactobacillus sp. in healthy Ossimi lambs. Polish Journal of Veterinary Sciences. 2018; 21(4): 705–713. https://doi.org/10.24425/124309
109. Shao Y. et al. Effects of Dietary Supplementation of Bovine Lactoferricin on Rumen Microbiota, Lactation, and Health in Dairy Goats. Frontiers in Nutrition. 2021; 8: 722303. https://doi.org/10.3389/fnut.2021.722303
110. El-Sharawy M.E. et al. Using lactoferrin and N-acetylcysteine to augment the growth rate and hemato-biochemical parameters of Egyptian Baladi goats kids. Cogent Food & Agriculture. 2024; 10(1): 2351041. https://doi.org/10.1080/23311932.2024.2351041
111. Teraguchi S., Ozawa K., Yasuda S., Shin K., Fukuwatari Y., Shimamura S. The Bacteriostatic Effects of Orally Administered Bovine Lactoferrin on Intestinal Enterobacteriaceae of SPF Mice Fed Bovine Milk. Bioscience Biotechnology, and Biochemistry. 1994; 58(3): 482–487. https://doi.org/10.1271/bbb.58.482
112. Robblee E.D. et al. Supplemental Lactoferrin Improves Health and Growth of Holstein Calves during the Preweaning Phase. Journal of Dairy Science. 2003; 86(4): 1458–1464. https://doi.org/10.3168/jds.S0022-0302(03)73729-1
113. Prenner M.L., Prgomet C., Sauerwein H., Pfaffl M.W., Broz J., Schwarz F.J. Effects of lactoferrin feeding on growth, feed intake and health of calves. Archives of Animal Nutrition. 2007; 61(1): 20–30. https://doi.org/10.1080/17450390600973675
114. Wang Y.-Z., Shan T.-Z., Xu Z.-R., Feng J., Wang Z.-Q. Effects of the lactoferrin (LF) on the growth performance, intestinal microflora and morphology of weanling pigs. Animal Feed Science and Technology. 2007; 135(3–4): 263–272. https://doi.org/10.1016/J.ANIFEEDSCI.2006.07.013
115. Ma X. et al. Effects of dietary supplementation of bovine lactoferrin on growth performance, immune function and intestinal health in weaning piglets. BioMetals. 2023; 36(3): 587–601. https://doi.org/10.1007/s10534-022-00461-x
116. Jahan M. et al. Dietary lactoferrin supplementation to gilts during gestation and lactation improves pig production and immunity. PLOS One. 2017; 12(10): e0185817. https://doi.org/10.1371/journal.pone.0185817
117. Shan T., Wang Y., Wang Y., Liu J., Xu Z. Effect of dietary lactoferrin on the immune functions and serum iron level of weanling piglets. Journal of Animal Science. 2018; 85(9): 2140–2146. https://doi.org/10.2527/jas.2006-754
118. Sarkar V.K. et al. Early-Life Intervention of Lactoferrin and Probiotic in Suckling Piglets: Effects on Immunoglobulins, Intestinal Integrity, and Neonatal Mortality. Probiotics and Antimicrobial Proteins. 2023; 15(1): 149–159. https://doi.org/10.1007/s12602-022-09964-y
119. Dierick M., Ongena R., Vanrompay D., Devriendt B., Cox E. Exploring the modulatory role of bovine lactoferrin on the microbiome and the immune response in healthy and Shiga toxin-producing E. coli challenged weaned piglets. Journal of Animal Science and Biotechnology. 2024; 15: 39. https://doi.org/10.1186/s40104-023-00985-3
120. Hu W., Zhao J., Wang J., Yu T., Wang J., Li N. Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets. Biochemistry and Cell Biology. 2012; 90(3): 485–496. https://doi.org/10.1139/o2012-003
121. Li Q. et al. Supplementation transgenic cow’s milk containing recombinant human lactoferrin enhances systematic and intestinal immune responses in piglets. Molecular Biology Reports. 2014; 41(4): 2119–2128. https://doi.org/10.1007/s11033-014-3061-5
122. Cooper C.A., Nelson K.M., Maga E.A., Murray J.D. Consumption of transgenic cows’ milk containing human lactoferrin results in beneficial changes in the gastrointestinal tract and systemic health of young pigs. Transgenic Research. 2013; 22(3): 571–578. https://doi.org/10.1007/s11248-012-9662-7
123. Badr H., Nabil N.M., Tawakol M.M. Effects of the prebiotic lactoferrin on multidrug-resistant Escherichia coli infections in broiler chickens. Veterinary World. 2021; 14(8): 2197–2205. https://doi.org/10.14202/vetworld.2021.2197-2205
124. Rehman A., Behan A.A., Arain M.A., Zhou C. Iron-bearing lactoferrin a functional feed additive for poultry industry: a review on health benefits and nutritional advancement. World’s Poultry Science Journal. 2024; 80(4): 1171–1188. https://doi.org/10.1080/00439339.2024.2409453
125. Geier M.S. et al. The effects of lactoferrin on the intestinal environment of broiler chickens. British Poultry Science. 2011; 52(5): 564–572. https://doi.org/10.1080/00071668.2011.607429
126. Hung C.-M. et al. Porcine lactoferrin administration enhances peripheral lymphocyte proliferation and assists infectious bursal disease vaccination in native chickens. Vaccine. 2010; 28(16): 2895–2902. https://doi.org/10.1016/j.vaccine.2010.01.066
127. Chen H. et al. Bovine lactoferrin alleviates aflatoxin B1 induced hepatic and renal injury in broilers by mediating Nrf2 signaling pathway. Poultry Science. 2024; 103(12): 104316. https://doi.org/10.1016/j.psj.2024.104316
128. Chen H.-L. et al. Recombinant porcine lactoferrin expressed in the milk of transgenic mice protects neonatal mice from a lethal challenge with enterovirus type 71. Vaccine. 2008; 26(7): 891–898. https://doi.org/10.1016/j.vaccine.2007.12.013
129. Dong H. et al. Effects of Lactoferrin and Lactobacillus Supplementation on Immune Function, Oxidative Stress, and Gut Microbiota in Kittens. Animals. 2024; 14(13): 1949. https://doi.org/10.3390/ani14131949
130. Han Z.-S. et al. High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors. Protein Expression and Purification. 2007; 53(1): 225–231. https://doi.org/10.1016/j.pep.2006.11.019
131. Zhang J. et al. Expression of active recombinant human lactoferrin in the milk of transgenic goats. Protein Expression and Purification. 2008; 57(2): 127–135. https://doi.org/10.1016/j.pep.2007.10.015
132. Yu H. et al. The dominant expression of functional human lactoferrin in transgenic cloned goats using a hybrid lactoferrin expression construct. Journal of Biotechnology. 2012; 161(3): 198–205. https://doi.org/10.1016/j.jbiotec.2012.06.035
133. Budevich A.I., Bogdanovich D.M., Paitserau S.N., Kirykovich Yu.K. Cryostability of embryos of goat producers of human lactoferrin bioanalog. Scientific support for livestock breeding in Siberia. Proceedings of the V International scientific and practical conference. Krasnoyarsk. 2021; 84–90 (in Russian). EDN XGCKCQ
134. Rudak A.N., Herman Yu.I., Budevich A.I., Zaremba N.L. Zootechnical characteristics of goats producing bioanalog of human lactoferrin. Zootechnical Science of Belarus. 2020; 55(1): 171–178 (in Russian). EDN TSJQXX
135. Budevich A.I. et al. Fertilization ability of sperm and transmission of alien DNA to progeny from human lactoferrin gene primary goatsproducers. Zootechnical Science of Belarus. 2010; 45(1): 22–28 (in Russian). EDN WIACHF
136. Oryol N.M. The effectiveness of the use of recombinant lactoferrin for correcting biochemical irregularities in rats with experimental doxycycline-induced cholestasis and alloxan model of diabetes. Journal of the Belarusian State University. Biology. 2017; (2): 72–79 (in Russian). EDN YRWYRN
137. Chernousov A.D. et al. Neolactoferrin as a Stimulator of Innate and Adaptive Immunity. Acta Naturae. 2013; 5(4): 71–76. https://doi.org/10.32607/20758251-2013-5-4-71-76
138. Koblyakov A.V., Antoshina E.E., Gorkova T.G., Goldman I.L., Trukhanova L.S., Sadchikova E.R. Braking effect of human lactoferrin (neolactoferrin) on growth of transplantable tumor of the cervix in mice. Problems in oncology. 2012; 58(5): 668–673 (in Russian). EDN PDRFTP
139. Appel M.J. et al. Sub-chronic (13-week) oral toxicity study in rats with recombinant human lactoferrin produced in the milk of transgenic cows. Food and Chemical Toxicology. 2006; 44(7): 964–973. https://doi.org/10.1016/j.fct.2005.11.012
140. Cooper C.A., Maga E.A., Murray J.D. Production of human lactoferrin and lysozyme in the milk of transgenic dairy animals: past, present, and future. Transgenic Research. 2015; 24(4): 605–614. https://doi.org/10.1007/s11248-015-9885-5
141. Wang M. et al. Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows. Scientific Reports. 2017; 7: 10733. https://doi.org/10.1038/s41598-017-11462-z
142. Budevich A.I., Petruschko E.V., Ermolitsky V.N., Payterova O.V., Svirskaya A.A. Influence of interval milking of producing goats on physicochemical indicators and content of human lactoferrin in animal milk. Scientific notes of the educational institution «Vitebsk Order “Badge of Honor” State Academy of Veterinary Medicine». 2023; 59(2): 93–98 (in Russian). https://doi.org/10.52368/2078-0109-2023-59-2-93-98
143. Budevich A.I., Petrushko E.V., Bogdanovich D.M., Kuznetsova V.N., Kirikovich I.K. Influence of the season and lactation on the milk composition of goats-producers of biosimilar human lactoferrin. Bulletin of NSAU (Novosibirsk State Agrarian University). 2021; (1): 81–91 (in Russian). https://doi.org/10.31677/2072-6724-2021-58-1-81-91
144. Ochoa T.J. et al. Randomized controlled trial of lactoferrin for prevention of sepsis in peruvian neonates less than 2500 g. The Pediatric Infectious Disease Journal. 2015; 34(6): 571–576. https://doi.org/10.1097/INF.0000000000000593
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Rebezov Ya.M., Rebezov M.B., Medvedeva U.Yu. Prospects for the use of lactoferrin in animal husbandry. Agrarian science. 2026;1(2):40-59. (In Russ.) https://doi.org/10.32634/0869-8155-2026-403-02-40-59
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