Improving the efficiency of artificial insemination in high-yielding cows with immunological infertility
https://doi.org/10.32634/0869-8155-2026-404-03-65-74
Abstract
Relevance. Currently, in dairy farming, cases of infertility in highly productive cows are being diagnosed with increasing frequency. Accelerated selection requires a comprehensive analysis of reproduction in order to improve its efficiency.
Methods. The research was conducted and the results were tested on farms in the Moscow and Oryol regions. The studies involved 52 service bulls and 246 cows, 72 of which had been previously diagnosed with “immune infertility.”
Results. A new diagnostic protocol was developed, which improved the effectiveness of artificial insemination in highly productive, genetically valuable cows with a history of more than 6 unsuccessful insemination attempts over 8 or more months. The basis of the protocol is the individual selection of service bulls by titrating their native semen in the alloserum of each problem cow. For individual selection, we used bulls that had undergone a complete andrological examination and had no titers of sperm autoantibodies in their own blood serum. The development of the method included the preparation and execution of artificial insemination, and the determination of pregnancy via ultrasound examination.
Conclusions. A solution to the problem of immunological infertility in repeatedly unsuccessfully inseminated cows with no clinical gynecological pathologies is presented. Testing of this protocol resulted in pregnancy in 44.4% of cases in barren cows with a preliminary diagnosis of immune infertility.
Keywords
immunological infertility,
autoantibodies,
alloantibodies,
barrenness,
individual selection of sires,
insemination success
Supporting agencies: The scientific article was prepared as part of the research carried out under the state assignment for scientific research work No. FGGN-2024-0013 of the Federal State Budgetary Scientific Institution, L.K. Ernst Federal Research Center for Animal Husbandry
About the Authors
A. I. Abilov
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Akhmedaga Imash ogly Abilov, Doctor of Biological Sciences, Professor, Honored Scientist of the Russian Federation, Chief Researcher
60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132
N. A. Kombarova
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Nina Anatolyevna Kombarova , Candidate of Biological Sciences
60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132
V. V. Turbina
L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Victoria Vladimirovna Turbina, postgraduate student
60 Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132
References
1. Ustimenko A.V., Abilov A.I., Kozmenkov P.L. Postpartum deviations in cows and first-calf heifers and methods for their correction. Agrarian science. 2025; (10): 45–57 (in Russian). https://doi.org/10.32634/0869-8155-2025-399-10-45-57
2. Reshetnikova N., Eskin G., Kombarova N., Poroshina E., Shavyrin I. Baseline conditions and herd reproduction strategy with the increase of cattle lactation performance. Dairy and beef cattle farming. 2012; (4): 2–6 (in Russian). EDN OZJADN
3. Kharlap S.Yu., Rebezov M.B., Gritsenko S.A., Safronov S.L., Bobyleva I.V., Zhuravel V.V. Dynamics of reproductive qualities of cows depending on the productive longevity. Agrarian science. 2022; (7–8): 93–97 (in Russian). https://doi.org/10.32634/0869-8155-2022-361-7-8-93-97
4. Khaliullin N.A., Vafin I.A., Motorin O.A., Vokueva N.E. Dynamics of cattle numbers in agricultural enterprises of the Republic of Tatarstan. Upravleniye riskami v APK. 2023; (4): 53–63 (in Russian). EDN IFWCIS
5. Lazzari G., Colleoni S., Duchi R., Galli A., Houghton F.D., Galli C. Embryonic genotype and inbreeding affect preimplantation development in cattle. Reproduction. 2011; 141(5): 625–632. https://doi.org/10.1530/REP-10-0282
6. Gupta V.K. et al. Bovine reproductive immunoinfertility: pathogenesis and immunotherapy. Frontiers in Veterinary Science. 2023; 10: 1248604. https://doi.org/10.3389/fvets.2023.1248604
7. Vickram A.S. et al. Role of Antisperm Antibodies in Infertility, Pregnancy, and Potential for Contraceptive and Antifertility Vaccine Designs: Research Progress and Pioneering Vision. Vaccines. 2019; 7(3): 116. https://doi.org/10.3390/vaccines7030116
8. Sokolovskaya I.I., Milovanov V.K. Immunology of animal reproduction. Moscow: Kolos. 1981; 264 (in Russian).
9. Sokolovskaya I.I. Hematogenital barriers and reproduction. Immunology of reproduction. Works of 3rd International Symposium. Sofia: Bulgarian Academy of Sciences. 1978; 64–83 (in Russian).
10. Sokolovskaya I.I. The immune system regulator of reproduction. Zootechniya. 1994; (1): 24–26 (in Russian).
11. Vlasova A.N., Saif L.J. Bovine Immunology: Implications for Dairy Cattle. Frontiers in Immunology. 2021; 12: 643206. https://doi.org/10.3389/fimmu.2021.643206
12. Chernyshov V.P. Immunoandrology. Kyiv: Zdorov’ya. 1983. 192 (in Russian).
13. Kombarova N.A., Abilov A.I. Clinical examination of breeding bulls for the state of the immune system and blood biochemistry. Dairy and beef cattle farming. 2009; (3): 30–32 (in Russian). EDN KWQQZZ
14. Sokolovskaya I.I., Abilov A.I., Kochetkov A.A., Oyvadis R.N. Detection of spontaneous autoimmunity in bulls in cellular reactions. Doklady VASKhNIL. 1988; (12): 24–27 (in Russian).
15. Kombarova N.A, Abilov A.I., Eskin G.V. Monitoring of breeding bulls for the presence of sperm autoantibodies. International bulletin of Veterinary Medicine. 2008; (3): 49 (in Russian). EDN TKTWUJ
16. Behl J.D., Verma N.K., Tyagi N., Mishra P., Behl R., Joshi B.K. The Major Histocompatibility Complex in Bovines: A Review. ISRN Veterinary Science. 2012; 2012: 872710. https://doi.org/10.5402/2012/872710
17. Matzaraki V., Kumar V., Wijmenga C., Zhernakova A. The MHC locus and genetic susceptibility to autoimmune and infectious diseases. Genome Biology. 2017; 18: 76. https://doi.org/10.1186/s13059-017-1207-1
18. Lushova A.A., Zheremyan E.A., Astakhova E.A., Spiridonova A.B., Byazrova M.G., Filatov A.V. B-lymphocyte subsets: functions and molecular markers. Immunologiya. 2019; 40(6): 63–75 (in Russian). EDN OWNPNF
19. Kabbani M.S., Sergeeva T.B., Shchegoleva L.S. Cell-mediated cytotoxicity (phenotypes of CD8 and CD16) in immune response (review). Novye issledovania. 2021; (2): 36–43 (in Russian). EDN AHCHIX
20. Hunter A.G. Immunology and fertility in the bovine. Journal of Dairy Science Dairy Science. 1989; 72(12): 3353–3362.https://doi.org/10.3168/jds.S0022-0302(89)79498-4
21. Kamalov A.A., Okhobytov D.A. Changes in immunoglobulins level (antispermal antibodies of A and G classes) in patients with infertility associated with the therapy by prospermatogenic biostimulators. Medical Council. 2017; (13): 144–149 (in Russian).https://doi.org/10.21518/2079-701X-2017-13-144-149
22. Heidl G. Characterization of fertility related antisperm antibodies — a step towards causal treatment of immunological infertility and immuno-contraception. Asian Journal of Andrology. 2010; 12(6): 793–794.https://doi.org/10.1038/aja.2010.112
23. Gupta S. et al. Antisperm Antibody Testing: A Comprehensive Review of Its Role in the Management of Immunological Male Infertility and Results of a Global Survey of Clinical Practices. The World Journal of Men’s Health. 2022; 40(3): 380–398.https://doi.org/10.5534/wjmh.210164
24. Naz R.K. Antisperm Contraceptive Vaccines: Where We Are and Where We Are Going?. American Journal of Reproductive Immunology. 2011; 66(1): 5–12. https://doi.org/10.1111/j.1600-0897.2011.01000.x
25. Bozhedomov V.A. et al. Etiopathogenesis of autoimmune responses against sperm. Andrology and genital surgery. 2012; 13(4): 45–53 (in Russian).EDN PNMJAZ
26. Abilov A.I., Novgorodova I.P., Bilas Ya.A. Monitoring of the biochemical status of breeding bulls. Agrarian science. 2022; (7–8): 80–85 (in Russian). https://doi.org/10.32634/0869-8155-2022-361-7-8-80-85
27. Abilov A.I., Zaripov F.R., Dunin M.I., Pyzhova E.A. Content of endogenous hormones in the blood serum of milking breeds bullsproducers in the conditions of Tatarstan region. Agrarian science. 2020; (7–8): 44–48 (in Russian). https://doi.org/10.32634/0869-8155-2020-340-7-44-48
28. Abilov A.I., Novgorodova I.P., Nikanova D.A., Кombarova N.A., Кorneenko-Zhilyaev Yu.A. Nonspecific resistance of breeding bulls depending on breeds, intrabreed age differences and total protein level. Agrarian science. 2024; (7): 55–61 (in Russian). https://doi.org/10.32634/0869-8155-2024-384-7-55-61
29. Abilov A.I., Amerkhanov Kh.A., Yeskin G.V., Zhavoronkova N.V. Sperm production of modern selection sires and hematologic characteristics. Zootechniya. 2014; (10): 26–28 (in RussianEDN STXXGZ
30. Prasad A.S. Zinc is an Antioxidant and Anti-Inflammatory Agent: Its Role in Human Health. Frontiers in Nutrition. 2014; (1): 14. https://doi.org/10.3389/fnut.2014.00014
31. Suttle N.F. Copper deficiency in ruminants; recent developments. The Veterinary record. 1986; 119(21): 519–522. https://doi.org/10.1136/vr.119.21.519
32. González-Maldonado J., Rangel-Santos R., Rodríguez-de Lara R., García-Peña O. Effect of injectable trace mineral complex supplementation on development of ovarian structures and serum copper and zinc concentrations in over-conditioned Holstein cows. Animal Reproduction Science. 2017; 181: 57–62. https://doi.org/10.1016/j.anireprosci.2017.03.015
33. Yan Cheng C., Murk D.D. The blood-testis barrier and its implications for male contraception. Pharmacological Reviews. 2012; 64(1): 16–64.https://doi.org/10.1124/pr.110.002790
34. Jiang X.-H. et al. Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice. Asian Journal of Andrology. 2014; 16(4): 572–580. https://doi.org/10.4103/1008-682X.125401
35. Güney Saruhan B., Sağsöz H., Akbalık E., Ketani M.A., Erdoğan S. Distribution of CD68-, CD8-, MHCI- and MHCII-positive cells in the bull and ram testis and epididymis. Anatomia, Histologia, Embryologia. 2018; 47(4): 313–321. https://doi.org/10.1111/ahe.12354
36. Chiu W.W.-C., Chamley L.W. Clinical associations and mechanisms of action of antisperm antibodies. Fertility and Sterility. 2004; 82(3): 529–535. https://doi.org/10.1016/j.fertnstert.2003.09.084
37. Naz R.K., Menge A.C. Antisperm antibodies: origin, regulation, and sperm reactivity in human infertility. Fertility and Sterility. 1994; 61(6): 1001–1013. https://doi.org/10.1016/s0015-0282(16)56747-8
38. Tabarkiewicz J., Selvan S.R., Cools N. Autoimmunity in Reproductive Health and Pregnancy. Journal of Immunology Research. 2018; 2018: 9501865.https://doi.org/10.1155/2018/9501865
39. Fair T. The Contribution of the Maternal Immune System to the Establishment of Pregnancy in Cattle. Frontiers in Immunology. 2015; 6: 7.https://doi.org/10.3389/fimmu.2015.00007
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For citations:
Abilov A.I., Kombarova N.A., Turbina V.V. Improving the efficiency of artificial insemination in high-yielding cows with immunological infertility. Agrarian science. 2026;(3):65-74. (In Russ.) https://doi.org/10.32634/0869-8155-2026-404-03-65-74
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