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Optimization of the composition of protein supplements and their application in high-protein crispbread technology

https://doi.org/10.32634/0869-8155-2026-407-06-145-155

Abstract

Relevance. Plant proteins and protein supplements are playing an increasingly important role in food technology, contributing to the creation of functional and nutritionally valuable products. Using proteins from sunflower, pea, pumpkin, and rice not only improves the amino acid composition and technological properties of bakery products but also increases their biological value. Optimizing crispbread recipes with plant proteins contributes to the creation of products that comply with the principles of healthy eating and sustainable environmental management, while simultaneously reducing environmental and economic production costs.

Methods. The objects of the study were a protein composition consisting of sunflower, pumpkin, pea, and rice proteins, as well as crispbreads made using this protein composition. The obtained samples were examined according to GOST 9846-88, using GOST 5670-96, GOST 21094-2022, GOST R 54731-2011.

Results. The study showed that the addition of a plant protein composition to crispbread recipes has a positive effect on the technological properties of the dough and the organoleptic properties of the finished product. However, replacing more than 30% of the flour results in a deterioration in the dough’s rheological properties and the development of a specific flavor. Based on the experimental data, optimal dosages of the protein mixture were determined — 10% and 20% to ensure compliance with physicochemical standards and high consumer properties. The sample with a 20% flour replacement shows promise for the industrial production of functional foods, including dietary and sports nutrition products.

About the Authors

M. R. Salakhov
South Ural State University
Russian Federation

Maksim Radikovich Salakhov, Master of Biotechnology

76 Lenin Аve., Chelyabinsk, 454080



O. V. Zinina
South Ural State University
Russian Federation

Oksana Vladimirovna Zinina, Doctor of Technical Sciences, Professor of the Department of Food and Biotechnology

76 Lenin Аve., Chelyabinsk, 454080



E. A. Vishnyakova
South Ural State University
Russian Federation

Elena Aleksandrovna Vishnyakova, postgraduate student of the Department of Food and Biotechnology

76 Lenin Аve., Chelyabinsk, 454080



M. B. Rebezov
Gorbatov Research Center for Food Systems
Russian Federation

Maksim Borisovich Rebezov, Doctor of Agricultural Sciences, Professor, Chief Researcher

26 Talalikhin st., Moscow, 109316



References

1. Varivoda A.A., Keniyz N.V., Rebezov M.B. Development of evidence-based approaches to the design of targeted food products for gerodietary nutrition. Agrarian science. 2023; (4): 145–151 (in Russian).https://doi.org/10.32634/0869-8155-2023-369-4-145-151

2. Rebezov M. et al. Role of beetroot as a dietary supplement in food products: Review. Plant Cell Biotechnology and Molecular Biology. 2020; 21(57–58): 8–16.EDN UVQIOH.

3. Asenova B.K., Amirhanov K.Zh., Rebezov M.B. Production technology of functional foods-tuffs for environmentally disadvantaged regions. Trade and economic problems of regional business space. Collection of materials of the XI International scientific and practical conference. Chelyabinsk: South Ural State University. 2013; 313–316 (in Russian).EDN RFYCTH

4. Rustemova A.Zh., Rebezov M.B. The use of leguminous mixture for bakery products. Agrarian science. 2023; (8): 137-142 (in Russian).https://doi.org/10.32634/0869-8155-2023-373-8-137–142

5. Kharlap S.Yu., Gorelik O.V., Rebezov M.B., Belyaeva N.V. Modern technologies for the production of bread for healthy nutrition and its quality. Innovative technologies for processing and storing agricultural raw materials and food products. Collection of scientific papers for the 90th anniversary of the All-Russian Research Institute of the Refrigeration Industry. Moscow: All-Russian Research Institute of the Refrigeration Industry; Saratov: Amirit. 2020; 395–401 (in Russian).EDN UUPWCN

6. Zinina O.V., Pavlova Ya.S., Rebezov M.B., Chanov I.M., Nikolina A.D., Nurymkhan G.N. Development and examination of a cracker enriched with dietary fiber. Agrarian science. 2022; (9): 173–179 (in Russian).https://doi.org/10.32634/0869-8155-2022-362-9-173-179

7. Kupchak D.V., Chechenina S.V., Luk’yanova A.N., Snazinov M.A. Tendencies and perspectives of improvement of crispbread selection. Food Industry. 2026; (1): 122-125 (in Russian).https://doi.org/10.52653/PPI.2026.1.1.025

8. Skripko O.V. Crispy breads with protein-carbohydrate flour for healthy diet. International Research Journal. 2023; (9) (in Russian).https://doi.org/10.23670/IRJ.2023.135.41

9. Skvortsova O.B., Gar’kina P.K., Ponomareva Ye.I. Selection of a rational dosage of pumpkin seed powder in the formulation of crispbread. Khleboprodukty. 2020; (12): 50–51 (in Russian).EDN NZFTLD

10. Pyanikova E.A., Cheremushkina I.V., Kovaleva A.E., Bykovskaya K.I. The effect of apple powder on the consumption of crispbread. Proceedings of the Voronezh State University of Engineering Technologies. 2020; 82(1): 157–163 (in Russian).https://doi.org/10.20914/2310-1202-2020-1-157-163

11. Klyuchko N.Yu., Pozdnyakova D.A. Study on the technology improvement of bakery products enriched with a fish protein-mineral additive. KSTU News. 2022; 66: 103–111 (in Russian).EDN QHWXAW

12. Serimbetova Sh., Akhlan T., Izembaуeva A. Development of functional breads from plant-based components. Science, New Technologies and Innovations of Kyrgyzstan. 2025; (1): 16–19 (in Russian).EDN BSVLHO

13. Wang S., Zhao F., Wu W., Lyu L., Li W. Proteins from Blackberry Seeds: Extraction, Osborne Isolate, Characteristics, Functional Properties, and Bioactivities. International Journal of Molecular Sciences. 2023; 24(20): 15371.https://doi.org/10.3390/ijms242015371

14. Zhou X., Zhang C., Cao W., Zhou C., Zheng H., Zhao L. A Comparative Functional Analysis of Pea Protein and Grass Carp Protein Mixture via Blending and Co-Precipitation. Foods. 2021; 10(12): 3037.https://doi.org/10.3390/foods10123037

15. Langyan S., Yadava P., Khan F.N., Dar Z.A., Singh R., Kumar A. Sustaining Protein Nutrition Through Plant-Based Foods. Frontiers in Nutrition. 2021; 8: 772573.https://doi.org/10.3389/fnut.2021.772573

16. Moudgil K.D., Venkatesha S.H. The Anti-Inflammatory and Immunomodulatory Activities of Natural Products to Control Autoimmune Inflammation. International Journal of Molecular Sciences. 2023; 24(1): 95.https://doi.org/10.3390/ijms24010095

17. Kharisma V.D. et al. Mangostenone Bioactive Compound from Garcinia mangostana L. as Antiviral Agent via Dual Inhibitors Against E6 HPV 16/18 Oncoprotein through Computational Simulation. Research Journal of Pharmacy and Technology. 2023; 16(11): 5045–5050.https://doi.org/10.52711/0974-360x.2023.00817

18. Kuznetsova E. et al. Determination of antioxidants in herbal supplements by HPLC and X-ray electromagnetic field detector in a scanning electron microscope system. Journal of Microbiology, Biotechnology and Food Sciences. 2023; 13(2): e10248.https://doi.org/10.55251/jmbfs.10248

19. Sunadi et al. Hepatitis E Inhibited by Rosmarinic Acid Extract from Clove Plant (Syzygium Aromaricum) through Computational Analysis. Pharmacognosy Journal. 2023; 15(4): 518–523.https://doi.org/10.5530/pj.2023.15.112

20. Rio-Aige K. et al. A diet rich in fibre and vegetable protein during gestation and lactation shapes maternal immunity, intestinal microbiota and lipid metabolism. eBioMedicine. 2025; 117: 105784.https://doi.org/10.1016/j.ebiom.2025.105784

21. Náthia-Neves G., Alonso E. Optimization of the subcritical water treatment from sunflower by-product for producing protein and sugar extracts. Biomass Conversion and Biorefinery. 2024; 14(2):1637–1650.https://doi.org/10.1007/s13399-022-02380-w

22. de Oliveira Filho J.G., Egea M.B. Sunflower seed byproduct and its fractions for food application: An attempt to improve the sustainability of the oil process. Journal of Food Science. 2021; 86(5): 1497–1510.https://doi.org/10.1111/1750-3841.15719

23. Vartiya P. et al. Modeling and optimization of protein extraction from sunflower seed Cake: RSM and ANN-GA approaches. Journal of Agriculture and Food Research. 2025; 22: 102088.https://doi.org/10.1016/j.jafr.2025.102088

24. Qin N., Shuang Q., Bao X. Improvement of gel properties and molecular mechanism of modified sunflower meal protein gel by gellan gum. Food Chemistry: X. 2025; 30: 102979.https://doi.org/10.1016/j.fochx.2025.102979

25. Pöri P., Aisala H., Liu J., Lille M., Sozer N. Structure, texture, and sensory properties of plant-meat hybrids produced by high-moisture extrusion. LWT. 2023; 173: 114345.https://doi.org/10.1016/j.lwt.2022.114345

26. Sá A.G.A., Pacheco M.T.B., Moreno Y.M.F., Carciofi B.A.M. Processing effects on the protein quality and functional properties of cold-pressed pumpkin seed meal. Food Research International. 2023; 169: 112876.https://doi.org/10.1016/j.foodres.2023.112876

27. Pacheco A.F.C. et al. Impact of ultrasound on pumpkin seed protein concentrate hydrolysis: Effects on Alcalase, protein, and assisted reaction. Applied Food Research. 2023; 3(1): 100281.https://doi.org/10.1016/j.afres.2023.100281

28. Habib M., Singh S., Hanan E., Jan K., Bashir K. Optimization of enzymatic hydrolysis for obtaining antioxidant hydrolysates from pumpkin seed protein: Improvement of the physicochemical, structural and functional properties. Applied Food Research. 2025; 5(2): 101272. https://doi.org/10.1016/j.afres.2025.101272

29. Sarinho A. et al. Valorization of pumpkin-seed press-cake and hulls from agri-food waste streams. Food Chemistry Advances. 2025; 9: 101147.https://doi.org/10.1016/j.focha.2025.101147

30. Rexhepi F. et al. Chemical changes of pumpkin seed oils and the impact on lipid stability during thermal treatment: study by FTIR — Spectroscopy. Journal of Microbiology, Biotechnology and Food Sciences. 2022; 11(6): e5839.https://doi.org/10.55251/jmbfs.5839

31. Kassymov S. et al. Using of pumpkin and carrot powder in production of meat cutlets: Effect on chemical and sensory properties. International Journal of Psychosocial Rehabilitation. 2020; 24(4): 1663–1670.EDN RDTUES

32. Abilmazhinova B. et al. Study chemical and vitamin composition of horsemeat cutlets with addition of pumpkin. International Journal of Psychosocial Rehabilitation. 2020; 24(8): 7614–7621.EDN RKIUKF

33. Yang T. et al. The impacts of post-anthesis warming on grain yield and quality of double-cropping high-quality indica rice in Jiangxi Province, China. European Journal of Agronomy. 2022; 139: 126551.https://doi.org/10.1016/j.eja.2022.126551

34. Wang X. et al. Nitrogen Fertilizer Regulated Grain Storage Protein Synthesis and Reduced Chalkiness of Rice Under Actual Field Warming. Frontiers in Plant Science. 2021; 12: 715436. https://doi.org/10.3389/fpls.2021.715436

35. Manzoor A. et al. Rice bran: Nutritional, phytochemical, and pharmacological profile and its contribution to human health promotion. Food Chemistry Advances. 2023; 2: 100296.https://doi.org/10.1016/j.focha.2023.100296

36. Siripan T., Bunyatratchata A., Thammapat P., Bungthong C., Li H., Siriamornpun S. Enzymatic extraction improves functional and phytochemical attributes of rice protein from green and ripe grains. Food Chemistry: X. 2025; 32: 103329.https://doi.org/10.1016/j.fochx.2025.103329

37. Meza S.L.R. et al. Sustainable rice bran protein: Composition, extraction, quality properties and applications. Trends in Food Science & Technology. 2024; 145: 104355.https://doi.org/10.1016/j.tifs.2024.104355

38. Baeghbali V., Euston S.R., He X., Donetti M., Maklad O., Acharya P. High moisture extrusion based texturization and functional modulation of pea protein isolate through integration with cultivated beef. Food Hydrocolloids. 2026; 172(3): 112154.https://doi.org/10.1016/j.foodhyd.2025.112154

39. Lu Z.X., He J.F., Zhang Y.C., Bing D.J. Composition, physicochemical properties of pea protein and its application in functional foods. Critical Reviews in Food Science and Nutrition. 2020; 60(15): 2593–2605.https://doi.org/10.1080/10408398.2019.1651248

40. Ge J., Sun C.-X., Corke H., Gul K., Gan R.-Y., Fang Y. The health benefits, functional properties, modifications, and applications of pea (Pisum sativum L.) protein: Current status, challenges, and perspectives. Comprehensive Reviews in Food Science and Food Safety. 2020; 19(4): 1835–1876.https://doi.org/10.1111/1541-4337.12573

41. Wu D.-T., Li W.-X., Wan J.-J., Hu Y.-C., Gan R.-Y., Zou L. A Comprehensive Review of Pea (Pisum sativum L.): Chemical Composition, Processing, Health Benefits, and Food Applications. Foods. 2023; 12(13):2527.https://doi.org/10.3390/foods12132527

42. Zhu J. et al. Construction of a predictive model for pea protein solubility evaluation and screening of processing-specific varieties using machine learning after interpretable optimization. Journal of Food Composition and Analysis. 2025; 149: 108807.https://doi.org/10.1016/j.jfca.2025.108807

43. Akopyan G.S., Tikhonov S.L., Tikhonova N.V. Biological Activation of Baker’s Yeast and the Possibility of Enrichment of Bakery Products with Peptides. Health, Food & Biotechnology. 2022; 4(3): 49–58 (in Russian).https://doi.org/10.36107/hfb.2022.i3.s146

44. Kornen N.N., Kalmanovich S.A., Lukyanenko M.V., Vershinina O.L., Fedoseeva O.V., Viktorova E.P. Study of the effectiveness of the effect of vegetable food additives on the process of activation of bakery pressed yeast. News of universities. Food Technology. 2019; (4): 43–45 (in Russian).https://doi.org/10.26297/0579-3009.2019.4.11


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For citations:


Salakhov M.R., Zinina O.V., Vishnyakova E.A., Rebezov M.B. Optimization of the composition of protein supplements and their application in high-protein crispbread technology. Agrarian science. 2026;(6):145-155. (In Russ.) https://doi.org/10.32634/0869-8155-2026-407-06-145-155

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