Assessment of hybrid material for resistance to Verticillium dahliae klebhan with the application of phytoimmunity marker enzymes

The aim of the work is to assess the sustainability of breeding significant interline hybrids F₆ to V. dahlia, created in NIESSVAH as a starting material for breeding. 21 samples were selected by the NIISSVAH staff and the Institute of Bioorganic Chemistry ANRUz and transferred for the relevant biochemical analysis. In the experiment, they determined the activity of peroxidase, then they revealed its activity in seedlings of cotton seeds, and also established responsiveness to the defeat of V. dahlia. As a result of the research, the seeds of 21 different cotton hybrids were tested to study the activity of the chitin-specific peroxidase enzyme. For this purpose, seedlings of families of hybrid combinations of cotton were used in order to select a breeding material with high resistance to Verticillium. In the work on the assessment of resistance to V. dahlia, biochemical tests were used using phytoimmunity marker enzymes - chitin specific peroxidase. As a result of a biochemical study in the laboratory, it was established that the complex interlinear combination F₆[F4-L-105 x L-108) x L-104] should be attributed to the pathogen-resistant V. dahliae among the studied samples. The family isolated from the F₆ hybrid [F₄(L-105xL-106)xL-106] was used as a parental family when breeding a new variety of cotton C-6577, which was transferred for study to the State test from 2018.

сорт, фермент, пероксидаза, хлопчатник, гибрид, устойчивость, вертициллиозный вилт, variety, enzyme, peroxidase, cotton, hybrid, resistance, verticillios wilt