Analysis of identity of genes of resistance to broomrape race G in some sunflower lines F₁

In the past few decades, the race formation of broomrape, a harmful obligate parasite of sunflower, has been increasing due to noncompliance with crop rotation standards. Over the course of several years, a number of inbreeding lines of various origin have been developed. They showed immunity to one of the last broomrape races, race G. The resistance control in these lines is monogenic with incomplete dominance. To be able to combine different genes in one sunflower genotype it is necessary to determine the allelism of resistance genes in the developed lines. Material and methods The sunflower lines served as the research material; it were the donors of resistance to the broomrape race G: RG, RGP1, RGP2, RGВ, RGL1, RGL2, RGM. The seeds of the broomrape race G were collected in the fields of Bokovsky and Morosovsky districts of the Rostov region. Seven hybrids of first generation from crossbreeding lines with each other were obtained in greenhouse and in the artificial climate chamber Biotron-5. Forced self-pollination was carried out by the method customary in VNIIMK, using individual spunbond insulators. Hybridization was conducted by means of manual castration. The plants were tested in the greenhouse for resistance and susceptibility to broomrape, using the early diagnosis method of A.Ya. Panchenko. Results As a result of the research, it was found that the manifestation of the resistance trait in F₁ plants is the same as in their parent forms. This type of inheritance of resistance in F₁, that is, the manifestation of the trait as in both parents, is typical for lines with an identical gene. However, the presence of two different dominant resistance genes would show the same result in the first generation. Since the resistance trait is dominant in the studied lines, the analysis of F₂ progeny will allow to confirm is the resistance gene identical or not for the lines RG with RGP1, RG with RGM, RG with RGL1, RG with RGB, RGP2 with RGL2, RGM with RGL1, RGB with RGL1.

подсолнечник, заразиха Orobanche cumana, устойчивость, раса G, тест на аллелизм, F₁, sunflower, broomrape, Orobanche cumana, resistance, race G, test for allelism, F₁