Porcine circovirus associated diseases diagnosis at industrial pig farm conditions

Porcine circovirus associated diseases (PCVAD) - one of the most economically significant diseases of swine. No one of the PCVAD has any specific clinical symptoms. Causative agent is a porcine circovirus type 2 which belongs to Circovirus genus Circoviridae family. Nowadays PCV-2 is ubiquitous. Vaccination against PCVAD is widely using in most of swine farms. Vaccine application against PCVAD does not elicit development of sterile immunity. These fact is making conduction od diagnostic investigations with using of materials from animals of different ages/technological groups are necessary. The article is describing results of investigation about PCVAD diagnosis with applying of longitudinal method of monitoring. Application of the ELISA test-kits for detection PCV- 2 specific immunoglobulins IgG/IgM and polymerase chain reaction for the DNA of PCV-2 detection in blood serum allowed to set significant differences in serology and virology profile in examined farms. It was founded that viremia level in different technological groups is ranged from 0 to 40%; seroprevalence level - from 0 to 100 and from 10 to 50% for specific IgG and IgM, respectively. Application of ELISA test-kit for detection of immunoglobulins IgM to PCV-2 allows to detect a contact animals with PCV-2 (or vaccination) earlier than in case with immunoglobulins IgG. The obtained data reveals possibility of application scientific-based approach in detection of major pathogen in case of systemic disease development such as porcine respiratory syndrome complex (PRDC). In addition, monitoring investigations might be helpful in case of study of a control measures and specific prevention of PCVAD.

мониторинг, цирковирус свиней 2 типа, IgG, IgM, распространенность, ДНК, monitoring, porcine circovirus type 2, IgG, IgM, prevalence, DNA