Differential cytophysiological markers of erythroid precursors, mature erythroid cells and shadows of erythroid cells in as a result of eryptosis in organism of animals

Relevance. Differential diagnostic markers of intact polychromatophilic erythroblasts, mature erythrocytes and erythroblast ghosts, and erythrocyte ghosts in normal peripheral blood are presented in model organism of animals — birds (Aves).

Methods. Morphophysiological characterization of erythroblasts, erythrocytes and erythroblast shadows, and erythrocytes shadows was performed using blood smears of Gallus gallus L. birds (n = 40, stained with Pappenheim) from four age groups (Postembryonalis — P1, P7, P23, and P42: days 1, 7, 23, and 42 of postembryonic ontogenesis) of a industrial herd. Identification and cytophysiological analysis shadows profile nucleus, shadows profile cytoplasm, shadows profile cell, and unidentified cell shadows were performed using calibrated high-resolution micrographs (n = 158).

Results. In a model organism of animals — birds — morphological changes in erythroblasts in the peripheral bloodstream as a result of eryptosis have a cytophysiological character consistent with the status of a clinically healthy animal. Apoptotic changes in blast and cellular forms of the erythroid component in the peripheral bloodstream of birds are grouped into nucleocytoplasmic formations of varying severity, including: cytolysis, cytoplasmic fragmentation, karyopyknosis, karyorrhexis, karyolysis, chromatin fragmentation, chromatinolysis, and cytoplasmic vacuolization. The study of eryptosis in the peripheral bloodstream of birds resulted in the development and formulation of differential criteria – markers of erythroid cell shadows – with clinical, general biological and didactic significance.

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