In this article we proposed a method for quantitative determination of active substance in pharmaceuticals via Raman scattering using potassium ferricyanide as internal standard. We approved this method in ketoprophenum quantitative determination in drug for veterinary use “Ketojekt”. The calculation of metrological requirements of procedures are established proposed method of calibration. Either we demonstrated the focalisation factor of laser beam affects on the method reproducibility. The method provides a precise, rapid, convenient to quantitative analysis that is more effective than the standard HPLC assay.

Topicality. To date, an extensive scientific and practical material has been accumulated to study the effectiveness of probiotic feed additives in the feeding of farm animals. Some mechanisms of their action on the animal body have been studied, positive effects have been established, which are expressed in improving the microbial balance of the intestine, increasing the protective properties of the body, and realizing the productive qualities of animals. It is necessary to study the effectiveness of the use of probiotic complexes in comparison with the action of antibiotic-containing drugs.

Methodology. It was studied the probiotic complex of Enzymsporin containing a consortium of bacteria of the genus Bacillus (Bacillus subtilis VKM B-2998 D, Bacillus licheniformis VKM B-2999 D, Bacillus subtilis VKM B-3057D) at least 5x109 CFU/g was studied in comparison with antibiotic-containing drug Virginiamycin in feeding fattened young pigs. The studies were carried out on four groups (30 heads in each group) of growing pigs in the conditions of LLC “Bryansk meat processing plant” and in the laboratories of L.K. Ernst Federal Science Center for Animal Husbandry. The duration of the experiment was 113 days.

Results. During the studies, the control group of animals received compound feeds without probiotic additives, while the animals of the 2nd and 3rd experimental groups received compound feed with the addition of probiotic complex in an amount of 0.5 and 1.0 kg/t, the 4th experimental group of pigs received compound feed with the addition of antibiotic-containing drug Virginiamycin 250 g/t. By the end of the study, the live weight and average daily increments of experimental animals increased by 5.0; 6.0; 10.02 and 5.8; 6.5; 12.2% (p < 0.001), respectively, compared with the control. Biochemical, morphological, immunological blood indices corresponded to physiological norms, the inclusion in the feed of pigs fed different levels of probiotic complex has led to a significant increase lysozyme, bactericidal and phagocytic activity of blood in animals 2nd and 3rd groups during rearing. Additional profit for the period of research was in the experimental groups of fattened young pigs +705,0; +849,0 and 1440.0 rubles/ head, accordingly, in comparison with the control, which indicates the effective use of not only antibiotic-containing drugs in feeding pigs, but also suggests the effective use of probiotics instead of antibiotic-containing feed preparations.

As a result of achievements in genetics and in technology of artificial insemination` cattle breeders received a powerful instrument of improving animals productive qualities, the possibilities of selecting stallions with a high genetic potential of productivity were expanded` and the rate of genetic improvement of entire populations was accelerated. Sperm from stallions is taken on an artificial vagina. Evaluation of the quality of sperm is carried out for 3 consecutive days. Insemination of mares is carried out at artificial insemination stations. 10 days after ovulation the mares are checked again with a probing stallion. If the mare for 35–40 days has not shown signs of sexual hunting again then it is followed by rectal test for foal.

Relevance. In Azerbaijan, the roots of sericulture lead to ancient times. The problem of obtaining new silkworm hybrids capable of adapting most quickly to adverse agroclimatic factors while maintaining high productivity is very relevant at the present stage of development of the agricultural sector of Azerbaijan.

Methods. The purpose of the study is to create silkworm hybrids Nesa2\1 x Khayal and Mizuri-5 x Mayak-6, which are superion in adaptability and resistant to agroclimate and seasoned feeding (spring, summer, autumn). The studies were developed by the parameters of integratedassessment and adaptive selection.

Results. Accerding to environmental parameters, the arerge ectimate of the stability constant and the selection index, comparable indicators of the Mizuri-5 x Mayak-6 are quite high. using the method of adaotive selection and integrated assessment, the results of our research give us grounds to assert that the Mizuri-5 x Mayak-6 hybrids adapt even to favorable conditions and very tolerant seasoning. Ultimately, the viability of the silkworm has a value of 10% moisture, 20% temperature and 70% mulberry, which is the only food for the silkworm caterpillars, and with changes of this agroclimate, the silkworms functional indicators change. Thus, the new hybrids we have created are resistant to the fluctuations of agrocilimate and correlate to any seasonal feed.

Relevance. The compliance with zoohygienic norms and rules is a prerequisite for the biological safety of a livestock enterprise. Biosafety requirements have been tightened especially in connection with pandemics of ASF, avian influenza and other infections. Disinfection is one of the most important measures to maintain epizootological well-being, and the quality of disinfection directly depends on the effectiveness of the disinfectant used.

Methods. The methods of mathematical analysis were applied.

Results. The effectiveness of the use of disinfectant solutions based on the Chistobel complex disinfectant was confirmed. The disinfectant is effective for all types of disinfecting treatments at all objects of veterinary supervision.

Relevance. Small-scale farming on a private basis have some disadvantages. Many similar farms do not take timely measures to combat pest diseases, especially against helminthiasis pathogens. Echinococcosis was among the main helminth diseases in sheep in the Absheron Peninsula territories and the adjacent Khizi region too, where we conduct research. It’s widespread in Azerbaijan and greatly damaged sheep breeding.

Reesults. During study, we detected the species of E. granulosus in sheep at all study points in the Absheron Peninsula and Khizi. Thus, the relative prevalence of invasion was recorded in the villages Tudar (47,9%), Jeyranbatan (47,6%), Altyagach (46,5%), and some low the percentage of infection in the villages of New Yashma (20,6%), Hovsan (21,8%), Mushvigabad (25,0%). Hence, analysis of the echinococcosis causative agent shows spread of this invasion throughout the farms in different forms. It has been studied in altitude belts too. There are such results: an invasion’s high extensiveness was recorded in the low-mountain (45,1%) zone.

Relevance. In plant growing practice plant growth regulators based on plant substances are widely used. Plant polysaccharides are among the biologically active compounds that can be used as natural raw materials to create new drugs.

Methods. The growth-promoting activity of galactomannans isolated from the seeds of the plants Crotalaria alata, Gleditsia triacanthos and Crotalaria sp. was studied on cotton and wheat crops when they were grown under saline conditions. The range of studied concentrations was in the range from 0.1% to 0.0001%. Presowing treatment was carried out for 18 hours, after which the seeds were germinated in Petri dishes with the addition of 1% NaCl saline solution at a temperature of 25 °C. The biological activity of polysaccharides was evaluated by the linear growth of aboveground and underground organs of seedlings. The results were statistically processed using the Original Pro program.

Results. The studied substances have growth-stimulating activity, which depended on their origin and concentration. So galactomannan obtained from Crotalaria alata, under salinization conditions, had a positive effect on the growth of wheat root at 0.01% concentration, and from Gleditsia triacanthos at lower concentrations, 0.001% and 0.0001%. On the stem growth, the maximum effect was exerted by a substance isolated from Crotalaria alata in 0.01% and 0.001% doses. On cotton, stem growth was activated by the polysaccharide Crotalaria sp. in 0.0001% concentrations, the root is galactomannan Gleditsia triacanthos in 0.01% concentration.

Relevance. Trace elements are of great importance in plant life. Due to their catalytic action, they allow plants to more effectively use the main elements of nutrition-solar energy, water and macronutrients — nitrogen (N), phosphorus (P) and potassium (K), which in turn has a positive effect on plant productivity and crop quality. Rape is very sensitive to the lack of boron, zinc. Trace elements are currently presented in various forms, and therefore the purpose of the research was to identify the effectiveness of boronand zinc-containing trace elements in the processing of seed material.

Methods. Theobjectofstudyisspringrape(Brassicanapus) variety Yubileiny. Field experiments and studies were carried out in 2015–2017 in the Birsky district of the Republic of Bashkortostan. Tested options using micronutrient fertilizers: zinc sulfate, 21.5%; Ultramag chelate Zn, 15%; boric acid, 17.5%; Ultramag doron, 11%.

Results. According to the results of studies, an increase in field germination by 6.5%, the yield of oilseeds by 37.1% and oilseeds by 1.7% when treated with Ultramag chelate Zn at a dose of 1 kg/ha was found.

Methods. A scientific study on the cultivation of remontant varieties of garden strawberries and on their basis planting material was carried out at the experimental field of the Tashkent State Agrarian University of the Republic of Uzbekistan in 2018–2019. The experiment involved such varieties of garden strawberries as Bountiful, Redgauntlet, Cobra, Tarpan, Avis Delight, Evie 2, which were grown both in open and protected soils. Studies have revealed that most varieties of garden remontant strawberries have the weak whiskers formation ability.

Results. However, when growing a mother plantation in a condition of greenhouse, the ability of intensive formation of whiskers, their growth and rooting of rosettes, in comparison with the open ground increases two to three times and can reach 25–28 pieces per individual plant. The average yield of berries in the mother bush plantation depending on the variety used when growing plants in the greenhouse can reach 3.2–3.4 kg/m2.