VETERINARY MEDICINE
At the present time, reconstructive surgeries are the primary treatment approach, which allow to effectively restore the destroyed area of the stroma, which resulted from deep corneal ulcers. Despite the presented variety of synthetic and biological materials used for the regeneration of the pathological area, further study and search for available, biocompatible materials capable of providing sufficient transparency in the postoperative period remains an urgent task. The article presents the results of using amniotic membrane as a biomaterial for prosthetics of deep corneal ulcers and descemetocele in dogs. The study was carried out at the Department of Diseases of Small Domestic, Laboratory and Exotic Animals of the Federal State Budgetary Educational Institution of Higher Education "ROSBIOTEKH" and the INNOVET Veterinary Clinic. The article presents the results of a study of 26 dogs with ulcerative keratitis, admitted for an appointment over a 9-month period, and describes the results of a study of age, sex and breed predisposition to the development of corneal ulcers. In 11.54% of cases, surgical treatment was performed using amniotic membrane as a biotransplant to ensure successful regeneration of the ulcer defect. As a result of treating deep stromal corneal ulcers, it was possible to achieve regeneration with the formation of leukoma of varying degrees of transparency, preservation of vision and a good cosmetic effect in all described cases. The obtained results of the histological study confirm the effective integration of the amniotic membrane and the prospects of its use in reconstructive and restorative operations for deep ulcer defects.
Hypoxia is a frequent cause of hypoenergetic states, in which lipid hydrolysis increases, fatty acid synthesis increases, which leads to an increase in their concentration, as well as the concentration of ketone bodies, which are substrates of oxidation in tissues. The multiplicity of pathological changes in the body under hypoxia requires the search for effective antihypoxants, as which can be used extracts of black currant and medicinal raspberry.
The aim of the work was to study the peculiarities of metabolism of unesterified fatty acids and ketone bodies during antihypoxant loading on the background of acute hemic, histotoxic and hypoxic normobaric hypoxia.
The study was carried out on white rats. During 15 days the animals received intragastrically extracts of black currant, raspberry medicinal, a mixture of these extracts in the ratio 1:1 and cytochrome C, which was administered intramuscularly. The concentration of nonesterified fatty acids (NEFA) was determined in serum, heart and brain tissues of rats, and ketone bodies — in serum. The increase in the concentrations of FAFAs and ketone bodies in all tissues under acute hypoxia of various genesis is an indicator of lipid metabolism disorders and failure of adaptation mechanisms. The introduction of antihypoxants contributed to the decrease in the concentration of fatty acids and ketone bodies in the tissues of rats, which indicates that the studied preparations have a high lipidoprotective and antioxidant effect, and the highest efficiency is demonstrated by a mixture of extracts of medicinal raspberry and black currant in the ratio of 1:1.
The aim of this work was to study the antimutagenic and DNA-protective effects of lipophilic cryofractions of bovine spleen and placenta in mice with mitomycin (MM)-induced genotoxicity. The effect of lipophilic cryofraction of bovine spleen (LCSC), lipophilic cryofraction of bovine placenta (LCPC) and their mixture in equal proportions (LCSPC) was studied. Outbred male mice were used in the experiments. The effects of a single administration of the substances separately and simultaneously with an intraperitoneal injection of MM were studied, as well as with three-time administration of the substances with an interval of 24 hours before the administration of MM relative to the negative and positive control groups. Afterwards, bone marrow samples were obtained from the animals for the micronucleus test and liver samples to determine the amount of mitochondrial DNA (mtDNA) damage in the 12S-16S and ND5 fragments. A single administration of LCSC, LCPC and LCSPC reduced the frequency of polychromatophilic erythrocytes with micronuclei (MNPCE) by 4.1, 3.9 and 2.4 times (p < 0.05), respectively, relative to the positive control group mice. With a course of administration of LCSPC, the frequency of MNPCE decreased by 31.3% (p < 0.05) relative to the positive control group. Administration of LCSC and LCSPC reduced the amount of mtDNA damage by 96.5 and 68.3% (p < 0.05), respectively, in the 12S-16S fragment relative to the positive control group mice. A course of administration of LCSPC before MM reduced the amount of mtDNA damage by 52.2% (p < 0.05) in the ND5 fragment. The antimutagenic effect of the studied cryofractions and the DNA-protective effect of LCSC and LCSPC in relation to mtDNA were revealed.
A scientifically of the effect of betulin-containing feed additive on the lysozyme and bactericidal activity of the blood serum of calves and dairy cows in the conditions of a livestock complex was carried out.
It was shown that after 14 days of the experiment, the lysozyme content in five-month-old calves of the experimental group was higher than in the control by 18.5% (p ≤ 0.01), in ten-month-old calves of the experimental group it was higher than the control by 23% (p ≤ 0.01), and in cows of the experimental group it exceeded the lysozyme content of the blood serum of cows of the control group by 35% (p ≤ 0.05). It was found that after 14 days of the experiment, the content of BASK in five-month-old calves of the experimental group exceeded the value of the control group by 22.5% (p ≤ 0.05), in ten-month-old calves of the experimental group it exceeded the data of the control group by 14.7% (p ≤ 0.01) and in cows of the experimental group by 36.75% (p ≤ 0.05) BASK was higher than in cows of the control group. Thus, the data obtained demonstrate the activation of nonspecific resistance factors in all studied age groups of animals after giving a feed additive containing betulin, and confirm the prospects of using a natural feed additive to correct the immune status of cattle.
ZOOTECHNICS
Relevance. The efficiency of cattle breeding is directly dependent on reproduction quality, where the condition of spermatozoa in breeding bulls plays a crucial role. One of the critical factors is DNA fragmentation in spermatozoa, which can cause genetic abnormalities and reduce the effectiveness of artificial insemination. Hormonal imbalance, particularly testosterone and estradiol levels, may affect DNA integrity. This study aims to investigate the influence of bulls’ hormonal status on DNA fragmentation in their spermatozoa.
Methods. The study was conducted on 43 Holstein bulls. DNA fragmentation was assessed using the alkaline comet assay, with a minimum of 250 spermatozoa analyzed per sample. Hormonal status (testosterone, estradiol, thyroxine) was evaluated through enzyme-linked immunosorbent assay (ELISA) of blood serum. Statistical analysis was performed using SPSS v.23 software, employing variance analysis and Student’s t-test.
Results. Over 98% of spermatozoa exhibited intact DNA, while moderate and severe damage was observed in 1% and 0.8% of cells, respectively. Bulls with intact DNA showed 62.3% and 72% higher testosterone levels compared to those with DNA damage (p < 0.05). Conversely, estradiol concentration was 40% higher in bulls with fragmented DNA. Correlation analysis revealed a weak but significant negative association between testosterone and DNA fragmentation (ρ ≈ -0.20) and a positive association between estradiol and DNA damage (ρ ≈ 0.18). Thyroxine levels showed no significant differences between groups.
Conclusions. High testosterone levels are associated with reduced DNA fragmentation, while elevated estradiol may contribute to its increase, potentially due to oxidative stress. These findings highlight the importance of hormonal balance in maintaining sperm DNA integrity in breeding bulls.
Relevance. The article presents the indicators of growth and development of the industrial population of sows of the maternal breed used for three-breed industrial crossbreeding. It was found that the sows of the mother breed selected for research according to parametric biostatistics have homogeneous values in terms of live weight, absolute, average daily, and relative gains in terms of body composition indices.
Methods. Purebred pigs of the Yorkshire mother breed (250 heads) were used as an object. Repair pigs were evaluated during bonification at the age of reaching a live weight of 100 kg, in sows these estimates were taken from the automated system of zootechnical accounting “Hybrid”.
Results. On average, the animals had a live birth weight of more than 1.2 kg and reached a live weight of more than 100 kg by the age of 108 days. The coefficient of variation in the sample under study is low and varies from 4.24 to 14.54%, which indicates the uniformity of the sample and its representativeness. The parametric criteria of the population (X, Mo, Me) in the selected group of pigs in terms of live weight in different periods of ontogenesis were actually at the same level.
The average age of reaching a live weight of 100 kg was 170.91 days.
Due to the uniformity of body weight indicators in different periods of ontogenesis, a similar pattern has been established in terms of absolute, average daily and relative weight gains of repair sows.
Based on the assessment obtained, depending on the gradations developed by the authors according to significant external characteristics of animals, the sow population was divided into 3 groups. The leading group included 103 heads (41% of the total livestock studied), 86 heads (35%) in the mediocre group, and 61 heads (24%) in the commodity group. The presence of the largest number of animals of an outstanding group in the population indicates the high genetic potential of the animals of the studied population.
The more dominant genes an individual has, the higher the degree of expression of the trait, and therefore the higher the activity of metabolic processes in the body, the intensity of the central nervous system, especially in terms of homeostasis, the emergence and propagation of electrical impulses in living cells, organs and organ systems.
The aim of the research is to develop a digital model for predicting the heritability of the level of wool productivity in the offspring of sheep based on bioenergetic parameterization of superficially localized biologically active centers.
The object of the research was yarkas and sheep, obtained from reproducing sheep of the North Caucasian breed by the method of heterogeneous age-diverse selection with bioelectric potentials of superficially localized biologically active centers of various sizes.
In the experiments, the limits of the coefficients of heritability of tonin, shearing of wool, natural length of wool, and live weight of animals were established. The level of bioelectric potentials (UBP) of sheep sheep can be successfully used as an additional criterion in the selection and selection of parental pairs and forecasting the productivity of future offspring. With a heterogeneous selection of bioenergetic compatibility of parent pairs of different ages, it was found that the best young were obtained from a combination of breeding rams with UBP PLBAC 43.9 mkA at the age of 1.5 years and queens with UBP PLBAC 41.2 mkA at the age of 3.5 years, as well as breeding rams with UBP PLBAC 49.6 mkA at the age of 2.5 years and queens with a 35.5 MK swimmer at the age of 1.5 years.
Relevance. Reproductive traits of goats are of high economic value. IGF2BP2 and BMPR1B genes were proposed as candidate genes associated with reproductive traits of goats. However, their role in the formation of multiple pregnancy in goats is poorly understood. In this regard, a more detailed study of polymorphism in the IGF2BP2 and BMPR1B genes may deepen the knowledge of the genetic mechanisms underlying multiple pregnancy in goats, so we chose these genes as targets for the experiment.
Methods. Complete genomes of goats of Orenburg (n = 15) and Karachaev (n = 15) breeds were obtained by whole genome sequencing performed by NGS. From whole genomes we extracted the complete sequences of candidate genes affecting the reproductive abilities of goats (IGF2BP2 and BMPR1B), in which a search for single nucleotide polymorphisms (SNP) was performed. The most different SNPs in the analyzed genes were selected based on the calculation of FST values for each SNP within each gene, performed using PLINK 2 software.
Results. Comparison of the nucleotide sequence of the IGF2BP2 and BMPR1B genes in Karachaev and Orenburg goats, the most different SNPs were identified based on the calculation of FST values. The greatest differences in sequences were found in the BMPR1B gene: 11 SNPs with FST values from 0.513305 to 0.596852. While in the IGF2BP2 gene, only 2 SNPs were identified (FST = 0.453078).
Understanding the genetic factors that determine the qualitative and quantitative characteristics of milk plays a crucial role in the development of highly productive dairy goat populations. In recent years, the use of genetic markers has become an integral part of breeding programs, allowing for a significant acceleration in the selection of animals with valuable production traits. This article provides an analytical review of current national and international studies aimed at identifying and characterizing candidate genes associated with dairy performance in goats. Particular attention is given to genetic markers linked to milk yield, composition, technological properties, and lactation persistency. Among the most significant genes are CSN1S1, CSN2, and CSN3, encoding casein proteins that strongly influence protein content and milk quality. Equally important are DGAT1 and DGAT2, which regulate triglyceride synthesis and fat content, as well as POU1F1, IGF-1, and PPARG, which control endocrine mechanisms of growth, metabolism, and lactation. In addition, a wide range of other candidate genes has been identified, involved in lipid and protein metabolism, cellular signaling pathways, and physiological processes underlying milk production. The analysis of data obtained in various breeds, including Saanen, Alpine, Toggenburg, Nubian, Karachai, and Chinese goats, highlights the relevance of molecular-genetic approaches in improving breeding programs. The implementation of marker-assisted selection in dairy goat husbandry will not only increase milk yield but also ensure high product quality, which is essential for the sustainable development of the industry.
AGRONOMY
Relevance. Breeding for drought resistance is one of the most important areas of maize breeding due to the increasing climate aridity and the location of maize crops in areas with unstable and insufficient moisture.
Methods. Field and laboratory trials were conducted at the “Agricultural Research Center “Donskoy” in 2011–2013, 2015–2023. Field trials were laid out according to the Methodology of Field Trials with Maize. The soil of the experimental plot was ordinary blackearth (chernozem), with a 140 cm of humus layer thickness. The climate was moderate continental with unstable moisture. The years of the study were characterized by different degrees of aridity (HTC 0.32–0.87). The objects of the study were 155 new constant self-pollinated maize lines. The estimation of drought resistance was carried out by the residual water deficit method.
Results. There have been identified fifty-six drought-resistant self-pollinated maize lines with low values of residual water deficit (RWD) in the flowering phase (4.6–13.3%) and in the milky- wax ripeness phase (6.8–14.2%). The RWD value depended not only on the lines, but also on the conditions of the year of the trial. There has been established a tendency for residual water deficit to increase in hot and dry years. There has been identified an average negative correlation (r = -0.44) between the value of the RWD in the flowering phase and the average daily air temperature in July; between the water deficit value in the milky-wax ripeness phase and the average daily air temperature in August (r = -0.65). There have been revealed low but reliable correlation coefficients (r = -0.24…-0.27) between the RWD and the amount of precipitation in different periods of maize development.
Relevance. The article presents the results of prolonged stress protection of spring wheat and barley crops of zoned varieties for the third agro-climatic zone of the Russian Federation. The research was carried out from 2018 to 2022, field experiments were conducted on gray forest soils of average fertility at an agricultural enterprise in JSC “Agrari” of the Ryazan region.
The purpose of the research is to experimentally substantiate the effectiveness of prolonged stress protection at all stages of preparation, storage and pre-sowing treatment of seed material, as well as during the growth and development phases of the plant, depending on weather conditions and phytotoxicity (inhibition of growth and crop damage by plants) of the soil.
Results. Screening of stress-resistant seed batches, stress-protective post-harvest storage and pre-sowing treatment with the Epin-Extra growth regulator activated germination of grain seeds and increased field germination by 5.3–8.2%. Plant treatment at IV–V and IX–X stages of organogenesis in grain crops (depending on the experimental variant) intensified the accumulation of terrestrial phytomass, the increase in the leaf index of crops and the net productivity of photosynthesis, respectively, by 13.5–16.9%, 17.1–23.1% and 7.1–7.7%. Prolonged plant protection due to stress resistance contributed to a stable increase in yields over the growing season, which averaged 20.0% for spring wheat and 15.8% for barley over an average of 5 years of experiments on soils with increased phytotoxicity. The crop growth occurred as a result of an increase in the productivity of the ear and the weight of 1,000 grains and was due to the consistent interrelation of applied stress-protective agricultural techniques throughout the growing season.
This research addresses the urgent issue of molecular genetic certification of hemp (Cannabis sativa L.) varieties, crucial amid rapid breeding progress and expanding industrial use. The aim of the research is a development a complex of SSR markers that will ensure reliable differentiation of hemp varieties for breeding and seed production practices and molecular genetic certification. The work presents the results of the analysis of twelve varieties of monoecious industrial hemp of the Central Russian ecotype using two sets of SSR markers: highly polymorphic dinucleotide markers and developed hexanucleotide microsatellite markers. The study included DNA extraction, PCR amplification, allele detection and statistical data processing. The results obtained showed an extremely high level of intravarietal polymorphism when using highly polymorphic markers, which complicates their use for genetic certification. The developed complex of 10 hexanucleotide SSR markers covering all chromosomes of the genome made it possible to carry out intravarietal differentiation of samples.
Ambrosia artemisiifolia is a weedy, invasive and allergenic plant of concern to agricultural producers worldwide. Ambrosia artemisiifolia is a weed, invasive and allergenic weed of concern to agricultural producers worldwide.
The purpose of this work is to analyze the distribution patterns of A. artemisiifolia during drought to recommend measures that effectively limit its spread.
Object of research — Ambrosia artemisiifolia. Agricultural fields, plots of steppe and piece planting in conditions of dryness and in its absence. During research, we used traditional methods of geobotanical inventory. Potential productivity calculated based on the number of female cotyledons before and after mowing. Hardness of soil was analyzed using DICKEY-john tester.
We found that Ambrosia artemisiifolia does not establish in steppe phytocenoses. On agricultural lands, its viability and seed productivity are significantly higher compared to artificial green spaces, whereas its population density is lower. The species’ occurrence frequency depends on the interplay of soil and climatic factors. During droughts in untreated open areas, soil hardness increases sharply; at values of 7 MPa or higher, A. artemisiifolia fails to develop, despite a substantial seed bank in the topsoil. Mowing does not significantly reduce its potential seed output. A high density of cereal crops suppresses the spread of A. artemisiifolia and mitigates soil degradation during dry periods.
In the current conditions of agricultural production, optimization of the feed base of domestic livestock through the development and implementation of innovative technologies for the production of high-quality feed, including effective methods of cultivating legumes using physiologically active substances, in particular, annual white sweet clover, is of paramount importance. In this regard, the aim was to identify the effect of the preparations “Mival Agro”, “Vitanoll N” and “Vitanoll RK” on biometric indicators and productivity of annual white sweet clover varieties Zavolzhsky and Srednevolzhsky. The research was conducted in 2020–2023 in the south of the forest-steppe zone of the Middle Volga region.
The object of research is the white annual sweet clover, the Middle Volga variety, and the Zavolzhsky variety. The use of physiologically active substances (FAV) has a positive effect on the agrobiological parameters of annual white sweet clover in the conditions of the Middle Volga region. It was found that treatment with “Vitanoll N” and “Vitanoll RK” preparations slightly prolongs the growing season, compensated by increased productivity. FAVS stimulate the growth and development of sweet clover, increasing plant height, foliage, green mass and dry matter content (“Vitanoll N” is a Middle Volga variety, “Vitanoll RK” is a Zavolzhsky variety). The use of “Mival Agro”, “Vitanoll N” and “Vitanoll RK” increases the yield of seeds, especially in the Zavolzhsky variety. The treatment of FAV affects the chemical composition of sweet clover, increasing the content of protein (“Mival Agro”, “Vitanoll RK”) and carotene (“Vitanoll N”, “Vitanoll RK”) in a completely dry substance. The results obtained indicate the prospects of using FAV to increase productivity and improve the quality characteristics of annual white sweet clover.
The article presents the results of a quality study of spring common wheat varieties and lines, including purple-grained forms, for the production of whole-grain bread. In view of the beneficial effect on human health of the consumption of whole-grain products, in particular wheat bread made from wallpaper flour, we set the goal to evaluate the quality of spring common wheat varieties and lines, including purple-grained ones, created at FRC Kazan Scientific Center RAS for the production of whole-grain bread. Grain parameters (protein and gluten content, its quality, hectolitre weight, vitreousness), rheological properties of dough (farinograph, alveograph), amylographic characteristics of flour and baking qualities were studied. The results showed that most of the varieties correspond to the 3rd quality class, while the Khazine variety and the purpule line of the Kk-21-09-6 high in protein and gluten. Rheological parameters of the dough obtained from whole grain flour, measured by a pharynograph and an alveograph, revealed significant differences in individual samples in terms of indic ators: the water absorption, the deformation energy (W), the maximum overpressure (P), the average abscissa to rupture (L). Amylographic analysis revealed significant differences in the viscosity of starch paste: in Khazine it is maximum (860 units), and in Kk-21-09-6 — it is extremely low (115 e. a.), which may indicate increased enzymatic activity of the latter. Baking tests have shown that the best volume and quality of bread are typical for Nadira and Khazine varieties, despite the differences in the rheological properties of the dough in these samples. Correlation analysis confirmed a weak relationship between traditional rheological tests and the final quality of bread baked according to standard GOST 27669-88, which underlines the need to develop specialized baking evaluation methods for whole grain flour.
The article presents the results of research on the study of the introduced apple variety Tsivt 11 on dwarf rootstocks M9 and on SK-7, SK-2, selected by the North Caucasus Federal Scientific Center for Horticulture, Viticulture and Winemaking. Based on the research results, biometric parameters of tree growth and development were determined, and the most productive combinations were identified.
The height of the trees in the garden, depending on the rootstock, on dwarf rootstocks SK-7 and M9 reached an average of 2.6–2.9 m, on the semi-dwarf SK-2 3.2 m, the diameter of the trunk was 6.6–7.7 cm, respectively, the length of one-year shoots varied from 47 to 63 cm.
Productivity is one of the main economic and biological properties of a variety, which reflects not only the degree of realization of the genotype, but also significantly depends on the biological characteristics of the rootstock, its adaptability to the natural and climatic conditions of the cultivation area. It has been established that the variety grafted onto the SK-7 rootstock has a yield that is 15% higher than on the M9 rootstock, and 4.3 times higher than on the semi-dwarf SK-2 rootstock, depending on the planting scheme. According to the structure of fruit formation, the variety is of the ring-type fruiting (70.3–86.3%). The fruits are of medium uniformity, round in shape, harmoniously sweet in taste with a fruit weight of 160–200 g (maximum — 230 g). As a result of studying the periodicity of fruiting, it was found that the highest early fruiting and regularity of the harvest was noted on the rootstock SK7, the periodicity index is 8.4%.
AGROENGINEERING AND FOOD TECHNOLOGIES
Relevance. Agro-food waste is a promising resource for the production of feed protein. Their processing using enzymatic hydrolysis and yeast cultivation allows obtaining microbial biomass with high nutritional value. This relevant area helps to solve the problem of feed protein deficiency in animal husbandry and sustainable use of resources in the context of environmental and economic challenges.
Methods. Component analysis of raw materials (sunflower husk, brewer’s grain, lupine, beet pulp) was carried out according to standard methods, the raw materials were crushed, dried, subjected to enzymatic hydrolysis using enzyme preparations of the Ferment Group of Companies (Republic of Belarus) at optimal pH and temperature, with control of sugar yield, pH and conversion. Yeasts S. cerevisiae and C. tropicalis were cultivated on the obtained hydrolysates and the potential for using agro-waste was analyzed.
Results. The maximum sugar yield was obtained during the hydrolysis of brewer’s grains (1.8%), for lupine — 1.7% (two peaks of sugar yield were observed at 2–4 and 7–9 hours), for pulp – 0.95% (peak at 4–5 hours). When cultivating S. cerevisiae and C. tropicalis on hydrolysates of brewer’s grains and lupine, the yeast grew more actively on brewer’s grains, reaching a protein content of 68.18% and 72.63% a. d. w. after drying, and using mainly the protein fractions of the original raw material dissolved in the hydrolysate during growth. The most promising hydrolysates are brewer’s grains and lupine, the optimal duration of enzymatic hydrolysis is 6–10 hours.
Nanosized selenium was synthesized by chemical reduction in an aqueous medium using ascorbic acid; the following substances served as stabilizers for selenium nanoparticles: sodium laureth sulfate, hydroxyethyl cellulose (B30K), catamine AB, bovine serum albumin, didecyldimethylammonium chloride, cocamidopropyl betaine, tween 80, methylcellulose (MC 100), and chitosan. The product was enriched at the rate of 30% of the daily dose of the essential microelement selenium per 1 liter of fermented milk product. The following physicochemical properties were measured for the test samples: active acidity of the medium and titratable acidity of the medium. Based on the data obtained, it is evident that the studied parameters differed slightly from the characteristics of the control sample. The active acidity of the fermented milk product medium with the addition of nanoparticles varied from 3.98 to 4.08, titratable acidity was in the range of 103–112 оТ with the control sample indicator of 116 оТ. Analysis of the obtained data showed that the antioxidant activity of the obtained fermented milk products is similar to the control and is at the level of 0.25 mg TE/ml, with the exception of samples enriched with nanosized selenium stabilized by surfactants — cocamidopropyl betaine and tween 80. These products had increased antioxidant activity, which was higher than the others by up to 20%. Thus, enrichment of fermented milk products with selenium nanoparticles can contribute to an increase in the antioxidant activity of the product without affecting its physicochemical parameters and quality characteristics.
Moisture transfer processes are the main reason for lowering the shelf life of glazed candies based on souffle-type whipped masses. To reduce the speed of such processes, the effect of gums with moisture-retaining properties on the formulation of sweets has been studied. The objects of the study were glazed candies based on souffle-type whipped masses, made with various types of gums (xanthan, guar, konjac) in an amount of 0.25%. The work carried out measurements of the mass fraction of moisture according to GOST 5900-2014 confectionery products. Methods for determining the mass fraction of moisture and dry substances and the indicator of water activity, GOST ISO 21807-2015 Microbiology of food and feed. Determination of water activity. Candy samples were packaged in a polypropylene (biaxially oriented) film with a thickness of 20 microns and stored in a Climacell 404 climate chamber (Czech Republic) to study the moisture transfer process at a temperature of 18 °C and a relative humidity of 40%. It has been revealed that the addition of gums in the manufacture of glazed sweets based on soufflé-type whipped masses can reduce the rate of moisture transfer by 1.2–3.2 times and increase the shelf life. It has been shown that for samples of sweets based on souffle-type whipped masses with the addition of xanthan gum, it is possible to increase the shelf life by 2–3 times. The results of the study can be used to substantiate and predict the shelf life of sugary confectionery products.
ISSN 2686-701X (Online)