Актуальность. Повсеместное нерациональное использование антибиотиков превратило устойчивость к противомикробным препаратам в глобальную проблему не только в ветеринарии, но и в здравоохранении, которая усугубляется прекращением разработки антибиотиков. Данное исследование поможет практикующим врачам оперативно принимать решения по выбору тактики антибактериального лечения еще до результатов лабораторной диагностики.

Цель исследования — проведение кросс-секционного исследования за 2023 год на территории Московского мегаполиса, направленное на определение наиболее распространенных условно-патогенных возбудителей в популяциях животных-компаньонов, выявляемых при болезнях различных групп органов, с одновременным исследованием явления антибиотикорезистентности и составлением списка антибактериальных средств с ранжированием по их эффективности.

Методы. Определение антибиотикочувствительности проводили дискодиффузным методом (ДДМ) в соответствии с МУК 4.2.1890-04 «Определение чувствительности микроорганизмов к антибактериальным препаратам».

Результаты. В результате исследовательской работы было проведено исследование образцов микробиологических посевов, взятых у животных-компаньонов (попугаев, кошек и собак домашнего содержания) на территории Московского мегаполиса и выделено 258 патогенов. Были определены наиболее встречаемые бактерии:

• из мочевыделительной системы животных наиболее часто выделяются Staphylococcus epidermidis и Escherichia coli;
• из желудочно-кишечного тракта наиболее часто выделяются грамотрицательные патогены с преобладанием штаммов Escherichia coli;
• при дерматологических заболеваниях животных чаще всего выделяются грамположительные бактерии с доминированием Staphylococcus spp.

Была исследована антибиотикорезистентность патогенов, а также проведено ранжирование антибактериальных препаратов по эффективности к определенным видам микроорганизмов

Relevance. Bacterial kidney disease (BKD, corineal renal infection, Di disease), the causative agent of which is the gram–positive non-spore motionless bacterium Renibacterium salmoninarum, is a common disease of salmon fish (Salmonidae), which causes significant damage to fish production in aquaculture and the development of the industry, as well as reduces the number of wild populations.

The purpose of this review was to analyze the epizootic situation of bacterial renal disease (BKD) among wild salmon and fish populations in aquaculture on the territory of the Russian Federation, as well as in other countries.

The article presents information on the incidence of BKD in the world and on the territory of the Russian Federation for the period 1933–2023, shows data on the spread of the disease among wild populations and farmed fish in aquaculture, describes possible transmission routes, native and suspected vectors of infection.

Methods. The authors consider measures to control the growth of the incidence of BKD in the world and on the territory of the Russian Federation. Due to the lack of an effective vaccine and a method of treatment, the most affordable method of controlling the spread of the disease is timely diagnosis. The article describes the methods developed for fast and effective diagnostics by the staff of the Laboratory of Ichthyopathology of the FSC VIEV: microagglutination reaction (PMA) and polymerase chain reaction in real time (real-time PCR).

Results. Unlike classical bacteriological methods, these reactions allow for the indication and identification of the causative agent of BKD within 24 hours.

Relevance. Equine laminitis is a multi-organ disease that can affect important systems of the horse’s body in the most neglected cases, but most often the hooves are affected, which in turn affects the economic component of the owner of the sick animal.

Methods. The work was performed on two groups of horses. The first group included clinically healthy horses, the second group had clinical signs of laminitis. Blood for tests was taken in compliance with a starvation diet and the rules of asepsis and antiseptics from the jugular. The calculation of the leukocyte formula was carried out manually. The results were systematically evaluated and compared with each other to calculate the percentage ratio.

Results. It was found that with laminitis there is a significant change in the cellular composition of the blood, in horses of the experimental group there is an increase in leukocytes (by about 302.5%), which may be associated with an inflammatory process in the body that triggers the active production of lymphocytes into the bloodstream. There is also an increase in segmented neutrophils when calculating the leukocyte formula (by 41.52%). This change is most likely caused by traumatic damage to the sole of the hoof. In this case, the increase in segmented neutrophils will be influenced by granulocyte colony stimulating factor (G-CSF), a hormone that stimulates the production and release of neutrophils from the bone marrow.

Relevance. Reproductive insufficiency of sows associated with circovirus infection is widespread in pig breeding enterprises. Therefore, an increase in the effectiveness of immunity formed in animals during the post-vaccination period is of crucial importance in the formation of the reproductive potential of sows.

Methods. The work was performed on sows, which at the end of the suckling period during weaning of piglets (on the 21st day after delivery) were vaccinated against CVS-2 with the “Ingelvak Circoflex” vaccine (Germany). In the experimental group, vaccination was combined with a two-time administration of a targeted immunobiostimulator “Transfer Factor” at a dose of 5 ml per head with an interval of 7 days (the second administration of “Transfer Factor” was carried out during vaccination). The effectiveness of post-vaccination immunity was evaluated based on the results of immunological, zootechnical and statistical studies.

Results. It was found that the formation of post-vaccination immunity in control group sows occurs under conditions of an increase in the concentration of immunoglobulins in the blood (∑G + M + A) by 1.25–1.59 times due to IgG and IdM, a decrease in the absolute number of T- and B-lymphocytes by 11.84–15.51% and 14.12–17.51% while maintaining their percentage share in the total pool of lymphocytes, a decrease in the level of T-helpers by 34.27–36.47 against the background of an increase in cytotoxic lymphocytes and killers by 4.47–66.67%, which determines the yield of piglets per farrow in the amount of 12.5 heads and stillbirth at the level of 9.67%. The combination of vaccination with the introduction of a specific targeted immunobiostimulator in sows of the experimental group determines in the post–vaccination period; an increase in the concentration of immunoglobulins in the blood by 24.93–71.56% due to IgG; a decrease in the number of T-lymphocytes by 19.50–23.76% against the background of an increase in B-lymphocytes by 20.00–21.25%; preservation of the absolute number of T-helpers and reducing the number of cytotoxic lymphocytes and killers by 2.14–3.00 times, contributing to a 3.24% reduction in stillbirth and an increase in the yield of piglets per farrowing to 13.0 heads. 

Relevance. Assessing the meat production of beef cattle to be transformed by involving an ever-increasing phenotypic data to improve the accuracy of genotype estimation of animals. This can be achieved through the use of live weight changes for individuals and feed consumption during the period of their rearing in feedlots. In this regard, the use of automatic feeding stations that record these parameters for a comprehensive assessment of the genotype of Aberdeen-Angus bulls is an important task of these studies.

Methods. Information by 212 individuals was used for Aberdeen Angus bulls that underwent final fattening lasting 60–65 days at GrowSafe feeding stations in the Bryansk region (Russia). Data on the slaughter quality of animals, the efficiency of feed using and the chemical composition of meat were studied. The integral value for dividing bulls into four comparison groups was the residual feed intake trait (RFI).

Results. The best combination of feed conversion rates (4.95 kg/kg), average daily gain (1.67 kg /day) and consumed dry matter of the diet (8.25 kg) during the fattening period at the site was shown by bulls with a negative RFI value and an increase above 1495 g relative to other comparison groups. The peers from the second group had a similar tendency, but their consumption of dry matter of feed was the highest (8.96 kg). The animals from the second and fourth comparison groups showed a higher moisture-retaining capacity of meat (60.87% and 60.39%) and the total amount of water-soluble antioxidants (0.147 mg/g and 0.148 mg/g) in it. Significant differences in the chemical composition of meat have not been established.

Relevance. Providing the population of the country with high–quality food products of its own production is the main task facing the employees of the agro-industrial complex of the country. One of these products is milk. The bulk of milk is obtained from cows of the dairy productivity direction. Currently, in the Sverdlovsk region, more than 75% of the total number of cattle belongs to the Holstein breed, created by the absorption of domestic black-and-white cattle by the Holstein. The study of economically useful signs of dairy cattle of the Holstein breed is relevant and has practical significance.
Methods. The research was carried out in breeding reproducers for breeding Holstein black-and-white cattle of the Sverdlovsk region. The evaluation included all cows that completed the first lactation in 2020-2021. Milk productivity was assessed by control milkings, milk quality indicators: MJ and MDB in milk in an average milk sample from each cow — once a month in the dairy laboratory of “Uralplemcenter” LLC.
Results. As a result of the conducted studies, it was found that the highest productivity indicators in cows were on the 3rd lactation — both for the entire lactation and for 305 days of lactation. Starting from the 4th lactation, there is a decrease in milk yields — from 0.3% (5th lactation) to 8.5% (8th lactation). Further, fluctuations in milk yields have been established in the direction of increasing or decreasing, which is explained by a decrease in the number of animals used in farms for a long time. Coefficient of reproductive capacity for all lactation (up to and including the 12th) stable and is 0.89–0.91, that is, there is a problem with the reproduction of breeding reproducers in dairy cattle herds. No relationship between reproductive functions and milk yield of cows has been established. 

Relevance. National sheep breeding is lagging behind other branches of animal husbandry considering using of modern DNA technologies. The search for new genes — potential candidates associated with economically significant traits — is relevant for a more complete disclosure of the genetic potential of sheep. An earlier genome-wide association study showed that the DGKH and PPP1R1C genes had a certain effect on body weight of sheep at birth and at the age of 90 days. In this regard, a more detailed study of polymorphism in the DGKH and PPP1R1C genes can deepen understanding of growth and development processes in domestic sheep, so we have chosen these genes as targets for the experiment.
Methods. Primers and probes were selected for amplification of a fragment with targeted SNPs in the DGKH and PPP1R1C genes with a length of 68 nucleotide pairs based on the reference DNA sequence on the 10th (NC_056063.1) and the 2nd chromosome (NC_056055.1) sheep represented in NCBI. To determine polymorphism, real-time PCR-based test systems were developed. Genotypes were determined using a multiparametric graph. The test systems were tested on 147 sheep of the southern meat breed.
Results. The developed test systems for promising DGKH and PPP1R1C genes allowed to clearly determine sheep genotypes based on using the PCR-RT. All three genotypes (T/T, C/T, and C/C) were identified in the DGKH gene. No homozygous genotype (T/T) was identified in the PPP1R1C gene in the studied population. The test systems are suitable for routine DNA analysis in molecular genetic laboratories.

Relevance. An actual approach to solving the problems of improving animal health and increasing productive indicators is the development of biologically active additives based on microbial consortiums of probiotic microorganisms that synthesize active metabolites that increase the selective benefits of the beneficial microflora of the gastrointestinal tract and its biological activity in the correction of microbiocenosis and metabolism.

Methods. The object of research was 5%, 10%, 15%, 20%, 25% sugar beet molasses. When cultivating in molasses, probiotic microorganisms Clostridium thermocellulociticus, Ruminococcus olbus, Clostridium lochheadii and Bifidobacterium bifidum strain 1 were used. A daily culture of microorganisms was used for inoculation on a nutrient medium, which was standardized to 1 × 10⁶ CFU/cm³.

Results. It has been established that the number of probiotic microorganisms that are part of the culture liquid during the cultivation of Clostridium thermocellulociticus, Ruminococcus olbus, Clostridium lochheadii on the eighth day in a 15% molasses-based nutrient medium is 9.9 × 10⁶ CFU/cm³. In studies during the cultivation of B. bifidum, a significant increase in the content of Histidine by 1.13 times, serine, leucine + isoleucine, methionine by 5.5–7.0 times, and other non-essential and conditionally essential amino acids was found, which indicates a significant activity of probiotic microorganisms in nutrient medium. A significant activity of the complex of microorganisms Clostridium thermocellulociticus, Ruminococcus olbus, Clostridium lochheadii was determined, an increase in amino acids was found: lysine and glycine by 3.39 times, leucine + isoleucine by 1.3 times, meteonine and valine by 2.36 times, proline by 7.5 times. 

Methods. For the first time, a comprehensive calibration model has been developed and presented for the rapid determination of basic near-infrared spectroscopy (IR) methods in feed amino acids, with application in the production of animal feed. The research principle is based on the Fourier equation for spectroscopy. In this work, Fourier methods in the Italian region (FTIR, FT-NIRS) were applied. The data obtained from the calibration models were confirmed using high-performance liquid chromatography. FT-NIR predictions agreed well with the chromatography data and had predictive deviation (RPD) values >1.3 in all cases.
Results. The results indicate that FT-NIR spectroscopy can be used as a simple and rapid tool for monitoring amino acids. In the course of the work, experimental confirmation of previously known facts was obtained — the possibility of visual separation of the spectra of not only counterfeit amino acids, but also the possibility of separation by L- and DL-optical isomers. The work shows that the discrepancies between the values obtained by the classical “wet chemistry” method and the values obtained from the constructed calibration models do not exceed the reproducibility limits of arbitration methods. A predictive model was used based on information flow elements using the OPUS/QUANT2 software package for multivariate calibration and construction of calibration models for amino acids. This chemometric analysis proved the fundamental possibility of determining amino acids in the product. It has been shown that the use of information channels opens up opportunities for the use of many chemometrics algorithms, including data preprocessing and the construction of predictive models.

Relevance. The use of phytosanitary means to ensure high and stable yields of winter wheat is one of the tasks of growing winter wheat. Field experiments were conducted in 2021 and 2022 at the Federal Research Center “Nemchinovka” in the central non-chernozem zone of the Moscow region.
Methods. The aim of the study is to evaluate the effect of various plant protection preparations on the productivity and quality of winter wheat varieties by intensity levels: basic (the purpose of using the biological potential of the variety by more than 50%), intensive (involving the use of intensive varieties and the creation of conditions for a more complete realization of their biological potential) and high–intensity (a set of measures aimed at ensuring the realization of the potential of the variety is more than 85%). Treatments included fertilizers, pesticides and growth regulators in various combinations and concentrations. 
Results. Three varieties of winter wheat were studied: Nemchinovskaya 85, Moskovskaya 40 and Moskovskaya 27. Insecticides (Picus 1.0 l/t, Danadim Power 0.6 l/ha, Picus 1.0 l/t + Danadim Power 0.6 l/ha, Picus 1.0 l/t + Vantex 60 ml/ha, Picus 1.0 l/t + Vincit forte 1.5 l/t and Picus 0.7 l/t + Vantex 50 ml/ha), fungicides (Impact Exclusive 0.75 l/ha, Alto Super 0.5 l/ha, Consul, CS 0.8 l/ha + Consul 1.0 l/ha and Consul, CS 0.8 l/ha), herbicides (Aton 0.06 kg/ha + Agroxon 0.5 l/ha + Foxtrot 1.0 l/ha, Exactly Extra 35 g/ha + Foxtrot 1.0 l/ha + Agroxon 0.5 l/ha, Tandem 0.03 kg/ha + Foxtrot 1.0 l/ha, Tandem 30 g/ha + Foxtrot Extra 0.4 l/ha + Agroxon 0.5 l/ha and Lintur 180 g/ha) have confirmed their effectiveness. Nemchinovskaya 85 increased yield by 1.14–3.10 t/ha, Moscow 27 — by 0.64–3.62 t/ha, Moscow 40 — 0,71–3,21 t/ha. 

Relevance. Currently, the study of the interaction of these traits with each other is a very important task of breeding. In the work, 15 varieties of grain sorghum were studied, differing in morphological parameters, growing season, degree of grain ripening, biochemical composition and yield of grain and green mass. 
The purpose of the study is to study the interaction of environmental conditions on the yield and biochemical composition of sorghum grain and to assess the significance of these relationships.
Methods. The studies were conducted in 2020, 2021 and 2022. The years of research differed in meteorological conditions (hydrothermal Selyaninov coefficient (SCC) was 0.56–0.76). To assess the biochemical composition in sorghum grain, the spectroscopy method was used using an infrared analyzer Spectra Star XT. The grain yield was determined according to the generally accepted method. 
Results. The experimental results were processed using the “Agros 2.09” software package by the method of correlation analysis. Based on the results of three years of research, a correlation was established between biochemical composition and the meteorological conditions of the year (sum of temperatures, total precipitation, SCC), as well as a significant effect of these traits on the yield of sorghum grain.

Relevance. An integral part in the creation of new varieties is the study of the elements of the crop structure of collection samples, and the determination of their impact on yield will allow the inclusion of samples with their optimal ratio in the breeding process.
Methods. The research was carried out in the fields of the “Donskoy” Research Center in 2019–2021 in accordance with the Methodology of the State Variety Testing (2019) and the Methodology of Field Experience (2012). The objects of research are 85 soybean samples from the world collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIGRR) of domestic and foreign breeding.
Results. The most productive were samples with plant height 55–70 cm, attachment height of the lower bean 16–17 cm and 20–21 cm, number of branches 1.0–1.5 pcs/rast, number of beans 30–35 pcs/rast, number of seeds 65–70 pcs/rast, weight of 1000 seeds 140–150 g and 190–200 g, weight of seeds from a plant 11–12 g, period vegetation period is 112–114 days, protein content is 39.0–39.5%, fat content is 21.0–21.5%.

Relevance. The study of test-plants potential consists in determining indicators that reflect the quality of the seed material and its required quantity during production.
Methods. The objects of research are modified biofungicides created on the basis of the microscopic fungus Trichoderma viride and natural minerals: diatomite, glauconite, sapropel, zeolite.
The evaluation of the characteristics of the test-plant (spring wheat variety Ulyanovsk 105) when treated with modified biofungicides was carried out on the basis of indicators as germination energy and seed germination, biometric data (seedling and root length) and biomass (seedling and root weight).
Results. It has been established that seed treatment with the created biofungicides has a positive effect on the germination energy (82.0–96.0%) and germination (87.0–96.0%) of the test plant under study. In all experimental variants, the length of the seedling was on average 11.0% higher compared to the control. The maximum values of this indicator were noted in the variants when the seeds of spring wheat were treated with a biofungicide based on T. viride (by 18.59%), as well as in the complex treatment of T. viride with a zeolite fraction of 0.04 mm (by 21.70%) and with diatomite (by 12.28%). Stimulation of the root system development by 8.71–21.13% was also noted. In all experimental variants, seed treatment with new biofungicides stimulates the formation of test plant biomass. The best values were noted in the variants with the treatment of T. viride and T. viride with zeolite (fraction 0.04 mm), where the weight gain of the seedling to the control is 25.67% and 20.86%, and the root weight is 25.23% and 18.39%, respectively. The use of modified biofungicides based on micromycete T. viride and natural minerals is promising for obtaining environmentally friendly crop products.

Relevance. Currently, one of the main tasks in forestry is to obtain high–quality planting material. Studies are being conducted on accelerated cultivation of coniferous and deciduous plants in forest nurseries with the use of growth stimulants that positively affect seed germination, the growth of biometric indicators, the safety and survival of plants. In this regard, the use of plant growth stimulants, which also include “Zircon” and “Ferovit”, is relevant.
Methods. The effect of root feeding with Ferovit and Zircon preparations on morphometric parameters of three-year-old Korean cedar seedlings (Pinus koraiensis Siebold et Zucc.) was studied. Venue: nursery of the Mountain Taiga Station — branch of the Federal Research Center for Biodiversity of Terrestrial Biota of East Asia FEB RAS in 2019—2022. Parameters such as the height of seedlings, height gain, root neck diameter, root length and biomass were studied.
Results. It was found that a significant effect is provided by a variant of root fertilization of seedlings with the use of “Zircon” and “Ferovit” at a concentration of 1.5 ml / 3 l of water solution. The indicators of the height of seedlings compared to the control increased by 126.1%, height increase — by 136.7%, root neck diameter — by 20%, root length — by 21.8%, biomass — by 252.1%.

Relevance. The study of the effect of preparations containing amino acids on the accumulation of free amino acids in plants is relevant in assessing their regulatory and anti-stress effects.
Methods. A laboratory experiment was carried out in 2020 on tomato plants of the Betta variety. Gray forest medium loamy soil was used as a substrate. The experiment was performed on soil with different salinity — a) control (the soil is not saline), b) saline soil (50 mmol/kg NaCl), c) saline soil (100 mmol/kg NaCl), using three feeding options: without top dressing, top dressing with “Sodium Glutamate”, top dressing with “Aminozol”. Amino acids were extracted from the roots and aboveground parts of plants in the vegetative growth phase with a mixture of “ethanol + chloroform + water” (12:5:2) and the method of high-performance liquid chromatography (HPLC) with pre-columnar derivatization of amino acids with phenylisothiocyanate was used. 
Results. Soil salinization with sodium chloride caused an increase in the amount of free amino acids in tomato seedlings. The total amino acid content increased to 849.8 µg/kg (50 mmol/kg NaCl) and 606.9 µg/kg (100 mmol/kg NaCl) compared to the control soil (385.3 µg/kg). Salinity contributed to the accumulation of serine (from 50.4 to 414.4 µg/kg) in seedlings. Treatment with preparations (“Monosodium glutamate” and “Aminozol”) affected the accumulation of a number of amino acids responsible for plant stress resistance. Supplementation with monosodium glutamate increased the total amino acid concentration to 1146.6 µg/kg (50 mmol/kg NaCl) and 1017.7 µg/kg (100 mmol/kg NaCl) compared to the corresponding variants without supplementation. At the same time, the content of glutamic (up to 188.3 and 425.1 µg/kg) and aspartic (up to 50.8 and 198.7 µg/kg) acids increased. “Aminosol” contributed to an increase in the amount of amino acids to 1834.2 µg/kg (50 mmol/kg NaCl) and 934.4 µg/kg (100 mmol/kg NaCl), respectively. 

Relevance. The transition to a grafted grape culture solved the problem of saving viticulture from phylloxera, however, the shortcomings of the grafted culture indicate that the problem has not been completely solved. The article substantiates the importance and possibility of maintaining a root culture of phylloxeratolerant varieties based on the development of innovative methodology and technological foundations for the creation and operation of intensive root plantations using scientifically based biotechnological and physiological methods against the background of phylloxera infection. Hypotheses are proposed and experimental data are presented confirming the possibility of forming root-related plantings of tolerant grape varieties in accordance with the biological characteristics of the grape plant. It has been established that the laying of new plantings with root-related cuttings of phylloxera-tolerant varieties in a permanent place using physiologically active compounds, meets the biology of the grape plant, promotes better plant development, accelerates the onset of fruiting and increases the fruitfulness of bushes, in comparison with traditional technology. 

Relevance. Annotation. Experimental data on improving the production technology of regulated enzymatic cleavage of chitosan to obtain its low-molecular derivatives are presented. Experimental studies were carried out in the conditions of the Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction of the Moscow State Academy of Veterinary Medicine and Biotechnology — MVA named after K.I. Skryabin and on the basis of the All-Russian Research and Technological Institute of the Biological Industry.
Methods. Acid-soluble chitosan from king crab shell (MM 700 kDa, SDA 85%), chitosan succinate (MM 330 kDa, SZ 75.2%), low-molecular food chitosan (hydrochloride) (MM 50 kDa) were used as raw materials for obtaining new modifications of chitosan, gel chitosan (2% solution in 2% acetic acid) manufactured by Bioprogress LLC.
Results. As a result of the experiment, the technological parameters of obtaining low-molecular chitosan by enzymatic hydrolysis were selected. The greatest decrease in the dynamic viscosity of chitosan was reliably observed in the pilot-industrial series II (by 29.3 times), and the pilot-industrial series I and III — by 6.9 and 10.6 times, respectively. As a result of enzymatic hydrolysis of chitosan in the pilot series II, it was possible to reduce the molecular weight from 700 to 24 kDa, and in series I and III — to 102 and 66 kDa. Based on the research results, a technological scheme for obtaining low-molecular-weight chitosan derivatives by enzymatic hydrolysis has been developed, tested and proposed for industrial use, technical conditions have been developed.

For the first time, system analysis and system synthesis of technological flows for the production of functional food products of a new generation were applied and element-by-element flow structures were worked out on a simulation model. The information obtained is systematized in accordance with the scheme of the functional structure of the search for a technical solution and used to create operator models.
For the first time, models of drinks for herodietic purposes have been developed, the technological flow has been worked out in production conditions. Information on nutritional and biological value was obtained, the safety of biologically active substances was established by the example of certain types of new beverages in the production and storage process.
Regulatory documentation for herodietic drinks has been developed and approved.
The creation of models and regulatory documentation for herodietic beverages, technological schemes of production and control will allow the production of herodietic products to be created at Russian enterprises. 
All drinks are produced in production conditions. 
The article presents the basic principles of the development of drinks for older generations, adequate to their needs. Formulas of multicomponent herodietic drinks based on grain and vegetable raw materials were developed using the principles of food combinatorics, oat broth was used as the basis, vegetable raw materials — carrots and pumpkin, and fruit — apples. 
Rationally constructed nutrition, especially for people over 60 years of age, helps to optimize the metabolic state of the body and increases the level of protection of the bodyʼs response to adverse environmental factors, therefore, the proposed beverage formulations are designed in accordance with the daily functional and physiological norm of the elderly.

Marshmallows are a popular confectionery among consumers. There is a high interest in the need to develop and change production technologies. One of the ways to expand the range of marshmallows is the use of glaze, which gives the products a special taste and aroma, protects the products from harmful effects that cause damage to the product. During storage, products coated with glaze made using lauric-type cocoa butter substitutes are prone to lipolytic spoilage with the appearance of an unpleasant soapy taste. The conducted studies have confirmed that the layer at the phase boundary between the glaze and the marshmallow mass is a source of contamination by mold fungi due to favorable conditions for the growth of microorganisms. It is shown that as the temperature increases, the amount of CMAFAnM in glazed marshmallows decreases from 2.1 × 10² CFU/g to 80 CFU/g, and the amount of mold increases from 130 to 170 CFU/g. Therefore, in order to ensure the safety of glazed whipped products, it is necessary to increase the requirements for the initial microbiological contamination of raw materials, storage conditions, and transportation. In addition, it is necessary to consider the possibility of introducing moisture-retaining components into the formulation to reduce the growth rate of microbiota in products.